[Cloning of the RIB7 gene encoding the riboflavin synthase of the yeast Pichia guilliermondii].

The RIB7 gene encoding the enzyme of the final stage of riboflavin biosynthesis in Pichia guilliermondii--riboflavin synthase was cloned on the pFL38 shuttle vector as the Sau3A fragment of the chromosomal DNA of about 4 kb. The HindIII fragment of 1.4 kb was subcloned from the hybrid plasmid pFR7 obtained onto the pUC18 plasmid.

[Cloning and inactivation of a chromosomal copy of the imidazole glycerophosphate dehydratase (HIS) gene from Hansenula polymorpha].

A gene for imidazole glycerophosphate dehydratase (HIS) has been selected from the library of Hansenula polymorpha genes by complementation of Escherichia coli hisB mutations or his3 mutation of Saccharomyces cerevisiae. Inactivation of the gene in Hansenula polymorpha strain 48V, as well as Leu(-)-Leu+ conversion of phenotype and arising of antibiotic G418 resistance resulted from transformation of the strain by the linear DNA molecules of the cloned HIS-gene with the in vitro inserted LEU2 gene from Saccharomices cerevisiae and KmR gene. The Southern hybridization analysis of the Leuf+His- G418R clones representing 1-2% of transformants population together with the DNA analysis of the monospore clones from the hybrid Leu+His-G418R transformant tetrad and tester strain have revealed the locus specific nature of the clones.

[Evaluation of the stability of algorithms in data processing by computerized diagnostic cardiocomplex ECG C3T-01].

Evidence is provided for the model of choosing cardiosignal depiction to estimate one of the most important parameters of an outfit operation - stability of the results of electrocardiosignal (ECS) processing. It should be mentioned that despite the fact that the given parameter is not so far generally acknowledged, it reflects accurately enough the operation "reliability" of the automated system. The author bases theoretically the choice of the adaptive piecewise model of ECS.

[Cloning of the RIB1 gene coding for the enzyme of the first stage of flavinogenesis in the yeast Pichia guilliermondi, GTP cyclohydrolase, in Escherichia coli cells].

The RIB1 gene encoding the enzyme of the first stage of the yeast Pichia guillermondii-GTP-cyclohydrolase- was cloned on pFL38 shuttle vector as the Sau3A fragment of chromosomal DNA of about 9 kb. EcoRI fragment of 4 kb with RIB1 gene was subcloned from the pFRI hybrid plasmid obtained into the pUC18 plasmid and then shortened to give 2.9 kb via deletion in SalGI site.

[A complex unit EKGS3T-01 for ECG recording and processing with preserving the syndromal findings].

Functional and technical parameters of the microprocessor diagnostic complex are being considered herein. It allows one to combine the automatic ECG data analysis and the possibility of the active participation in the processing of the cardiological information.