[Microglia detection in the brain preparations after long-term storage in formalin].

Relatively simple protocol for identification of microgliocytes in archived material is proposed. A novel variant is based on preparation processing with the use of polyclonal antibodies against Iba-1 protein. The proposed approach includes antigen heat unmasking in a modified citrate buffer and prolonged incubation of the sections with the primary antibodies at high temperature.