Engineered Fungus Producing Plant Storage Proteins.

An efficient -mediated genetic transformation based on the plant binary vector pPZP-RCS2 was carried out for the multiple heterologous protein production in filamentous fungus   F-859 (formerly   F-859). The engineered fungus   was able to produce plant storage proteins of   (α-zein Z19) and   (albumin A1) to enrich fungal biomass by valuable nutritional proteins and improved amino acid content. The mRNA levels of and genes were significantly dependent on their driving promoters: the promoter of tryptophan synthase (P) was more efficient to express , while the promoter of translation elongation factor (P) provided much higher levels of transcript abundance.

Managing activity and Ca dependence through mutation in the Junction of the AtCPK1 coordinates the salt tolerance in transgenic tobacco plants.

Calcium-dependent protein kinases (CDPKs) are Ca decoders in plants. AtCPK1 is a positive regulator in the plant response to biotic and abiotic stress. Inactivation of the autoinhibitory domain of AtCPK1 in the mutated form KJM23 provides constitutive activity of the kinase.

Medicinal mushrooms as an attractive new source of natural compounds for future cancer therapy.

Medicinal mushrooms have been used throughout the history of mankind for treatment of various diseases including cancer. Nowadays they have been intensively studied in order to reveal the chemical nature and mechanisms of action of their biomedical capacity. Targeted treatment of cancer, non-harmful for healthy tissues, has become a desired goal in recent decades and compounds of fungal origin provide a vast reservoir of potential innovational drugs.

Towards an advanced cell-based glioma model system.

The modulation of tumor growth and development has always been one of the key factors in the research of the malignant transformation, including gliomas, prevalent and most deadly cancers of the brain. Indeed, cellular and molecular biology research employing model cell-based systems have great potential to advance both the mechanistic understanding and the treatment of human glial tumors, as it facilitates not only the understanding of glioma biology and its regulatory mechanisms Additionally they promise to afford the screening of the putative anti-tumor agents and alternative treatment approaches in a personalized manner, i.e.