[IMMUNOLOGIC MONITORING OF HELICOBACTER PYLORI PERSISTENCE IN THE ORGANISM].

Generalized results of 15-year prospective studies of frequency of occurrence and dynamics of circulation of pathogenetically significant LPS/O-antigens, high molecular weight proteins, including CagA, and VacA of Helicobacter pylori in biological media of organism in patients with gastrointestinal diseases and asymptomatic volunteers due to effects of external and internal factors are presented. Features of antigen circulation and reciprocal immune reaction of the organism are established, that reflect their interaction in the parasite-host tandem, risk and prognosis of possible complications in the process of long-term persistence of Helicobacter pylori in the organism.

[Production of recombinant fragment of VacA protein and development of non-invasive method for the diagnosis of Helicobacter pylori infection].

Vacuolizing toxin (VacA) Helicobacter pylori is an important factor of pathogenicity of Helicobacter pylori and a basic marker in the diagnosis of helicobacteriosis and related diseases. A coagulation-based diagnostic test-system was elaborated for the detection of VacA in clinical samples. A fragment of vacA was cloned, for the purpose, in Escherichia coli and expressed in preparative quantities; the coded protein was purified and used in raising the diagnostic serum.

[Occurrence of Helicobacter pylori specific antigens in diseases of gastrointestinal tract].

The results of the immunophoretic analysis of H. pylori antigenic structure are presented. Along with H.