Publications by authors named "M S Steinke"
Cellular metabolism must adapt rapidly to environmental alterations and adjust nutrient uptake. Low glucose availability activates the AMP-dependent kinase (AMPK) pathway. We demonstrate that activation of AMPK or the downstream Unc-51-like autophagy-activating kinase (ULK1) inhibits receptor-mediated endocytosis.
View Article and Find Full Text PDFEcological theory and empirical research show that both direct lethal effects and indirect non-lethal effects can structure the composition of communities. While the direct effects of grazers on marine phytoplankton communities are well studied, their indirect effects are still poorly understood. Direct and indirect effects are inherently difficult to disentangle in plankton food webs.
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- The FDA's decision to remove mandatory animal testing for drug approval signifies a move towards alternative research methods, with growing support from the European Parliament for animal-free practices.
- In vitro tissue models are being embraced for regulatory assessments in line with the 3R principles, but barriers like standardization, availability, and cost still limit their use.
- The Robotic Enabled Biological Automation (ReBiA) system is designed to automate laboratory processes, improving consistency and cost-effectiveness of in vitro models, as demonstrated by successful production of human tissue models that closely resemble native tissues.
Head and neck squamous-cell carcinoma (HNSCC) is associated with aggressive local invasiveness, being a main reason for its poor prognosis. The exact mechanisms underlying the strong invasive abilities of HNSCC remain to be elucidated. Therefore, there is a need for in vitro models to study the interplay between cancer cells and normal adjacent tissue at the invasive tumor front.
View Article and Find Full Text PDFNasal xenobiotic metabolizing enzymes (XMEs) are important for the sense of smell because they influence odorant availability and quality. Since the major part of the human nasal cavity is lined by a respiratory mucosa, we hypothesized that this tissue contributed to nasal odorant metabolism through XME activity. Thus, we built human respiratory tissue models and characterized the XME profiles using single-cell RNA sequencing.
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