Antibacterial potential of complex cystic fibrosis isolates.

Unlabelled: Over 160,000 people worldwide suffer from cystic fibrosis (CF), a genetic condition that causes mucus to accumulate in internal organs. Lung decline is a significant health burden for people with CF (pwCF), and chronic bacterial pulmonary infections are a major cause of death. complex (Smc) is an emerging, multidrug-resistant CF pathogen that can cause pulmonary exacerbations and result in higher mortality.

Delayed-Incubation Method for Microbiological Analysis of Environmental Specimens and Samples.

Five laboratories compared the quantitative recovery of heterotrophic bacteria, yeasts and molds, coliforms, Escherichia coli , Pseudomonas aeruginosa , and Staphylococcus aureus from a variety of naturally contaminated food and water samples, using traditional incubation procedures and a delayed-incubation method. Refrigeration of inoculated media for up to 3 days prior to incubation was shown to be a viable option for many quantitative analyses, but needs to be validated for each application. Some inoculated media withstood refrigeration for up to 7 days prior to incubation without any detrimental effect on the recovery of target cells, while the incubation of other media for similar types of analyses could not be delayed even for 3 days.

Nitrous oxide constricts epicardial coronary arteries in pigs: evidence suggesting inhibitory effects on the endothelium.

Nitrous oxide increases peripheral vascular tone in animals and humans and constricts epicardial coronary arteries in dogs. Current studies sought both to determine whether nitrous oxide caused epicardial coronary artery constriction in a second animal species, pigs, and to investigate mechanisms. Left anterior descending coronary artery diameters were measured by using computer-assisted angiography in intact pigs anesthetized with ketamine and fentanyl.

Caloric requirements in patients with severe head injuries.

Indirect calorimetry was performed on 15 nonsteroid-treated, head-injured patients over 16 postinjury days. A search was made for a clinical marker that could be used to predict the caloric requirement in individual head-injured patients. Of all parameters reviewed, only motor activity correlated with the caloric needs of the patients (P less than 0.