[RNA editing. The classical cases and the outlook of new technologies].

This article provides an overview of studied RNA editing cases and the examples of RNA editing investigations that apply to different experimental data. Typical RNA editing site prediction errors and the methods to minimize them are shown. The outlook of up-to-date technologies and further RNA editing studies are discussed.

[Draft macronuclear genome of a ciliate Euplotes crassus].

Basic bioinformatical analysis of the draft Euplotes crassus macronuclear genome and transcriptome suggests that more than a quarter of E. crassus genes contain several exons. A large fraction of all introns is formed by "tiny" introns having length 20-30 bp.

[Machine learning study of DNA binding by transcription factors from the LacI family].

We studied 1372 LacI-family transcription factors and their 4484 DNA binding sites using machine learning algorithms and feature selection techniques. The Naive Bayes classifier and Logistic Regression were used to predict binding sites given transcription factor sequences and to classify factor-site pairs on binding and non-binding ones. Prediction accuracy was estimated using 10-fold cross-validation.

[Regulation of multidrug resistance genes by transcriptional factors from the BltR subfamily].

BltR is a MerR family transcriptional factor, experimentally characterized in Bacillus subtilis. It activates transcription of genes encoding multidrug transporter Blt and spermine/spermidine acetyltransferase BltD. Here we studied BltR dependent regulons in 25 bacterial genomes using the comparative genomic approach.