Psychometric Testing of the Protocol for Responding to and Assessing Patient Assets, Risks, and Experiences (PRAPARE) in Patients With Heart Failure and Coronary Heart Disease.

The social determinants of health (SDOH) have been recognized as an important contributor to an individual's health status. A valid and reliable instrument is needed for researchers and clinicians to measure SDOH. However, there is considerable variability in the screening methodologies, as well as a lack of standardization in definitions and methods for capturing and reporting SDOH data for both electronic health record software vendors and national experts on SDOH.

A consensus platform for antibody characterization.

Antibody-based research applications are critical for biological discovery. Yet there are no industry standards for comparing the performance of antibodies in various applications. We describe a knockout cell line-based antibody characterization platform, developed and approved jointly by industry and academic researchers, that enables the systematic comparison of antibody performance in western blot, immunoprecipitation and immunofluorescence.

Transmission of Cryphonectria Hypovirus 1 (CHV1) to and In Vitro and In Vivo Testing of Its Potential for Use as Biocontrol Against .

Cryphonectria hypovirus 1 (CHV1) is successful in controlling , the causal agent of chestnut blight, but little is known regarding its transmission to other fungi, for example the European . In this study, CHV1 was transmitted (circa 200,000-800,000 copies/microliter) to seven isolates from infected . Reverse transmission to virus-free (European 74 testers collection) was achieved, although it was less successful (250-55,000 copies/µL) and was dependent on the vegetative compatibility (VC) group.

Association of depressive symptoms and engagement in physical activity with event-free survival in patients with heart failure.

Background: Heart failure (HF) subtype, depressive symptoms, and physical inactivity independently contribute to survival outcomes, but the effect of the interaction of these variables on survival outcomes remains unknown.

Objectives: We aimed to determine whether depressive symptoms and engagement in physical activity differentially interact to predict the combined endpoint of all-cause death or rehospitalization among patients with HF and reduced (HFrEF) or preserved ejection fraction (HFpEF).

Methods: This study was a secondary analysis.

A guide to selecting high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot, immunoprecipitation, immunofluorescence and flow cytometry.

Synaptotagmin-1 is a synaptic vesicle transmembrane protein that senses calcium influx via its tandem C2-domains, triggering synchronous neurotransmitter release. Disruption to is associated with neurodevelopmental disorders, highlighting the importance of identifying high-quality research reagents to enhance understanding of Synaptotagmin-1 in health and disease. Here we have characterized thirteen Synaptotagmin-1 commercial antibodies for western blot, immunoprecipitation, immunofluorescence and flow cytometry using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls.