Effect of Vitis vinifera zygotic embryo cryopreservation and post-cryopreservation on the gene expression of DNA demethylases.

Grapevine (Vitis vinifera L.) crops are continuously exposed to biotic and abiotic stresses, which can cause genetic and epigenetic alterations. To determine the possible effects of grapevine cryopreservation on the regulation of DNA demethylase genes, this work studied the expression of DNA demethylase genes in cryopreserved and post-cryopreserved grapevine tissues.

Assessing metal(loid)s concentrations and biomarkers in tilapia (Oreochromis niloticus) and largemouth bass (Micropterus salmoides) of three ecosystems of the Yaqui River Basin, Mexico.

Aquatic ecosystems have been suffering deleterious effects due to the development of different economic activities. Metal(loid)s are one of the most persistent chemicals in environmental reservoirs, and may produce adverse effects on different organisms. Since fishes have been largely used in studies of metal(loid)s exposure, tilapia and largemouth bass were collected in three ecosystems from the Yaqui River Basin to measure the concentrations of metal(loid)s (chromium (Cr), copper (Cu), iron (Fe), manganese (Mn), nickel (Ni), zinc (Zn) arsenic (As), mercury (Hg), and selenium (Se)) and some biomarkers (somatic indices, metallothionein expression and histopathological analysis) in tissues of both species.

Transcriptome assessment in 'Red Globe' grapevine zygotic embryos during the cooling and warming phase of the cryopreservation procedure.

Cryopreservation has the potential for long-term germplasm storage. The metabolic pathways and gene regulation involved in cryopreservation procedures are still not well documented. Hence, the genetic expression profile was evaluated using RNA-Seq in zygotic embryos of grapevines subjected to cryopreservation by vitrification.

Functional analysis of tomato rhamnogalacturonan lyase gene Solyc11g011300 during fruit development and ripening.

Rhamnogalacturonan I (RG-I) is a domain of plant cell wall pectin. The rhamnogalacturonan lyase (RGL) enzyme (EC 4.2.

Functional analysis of a tomato (Solanum lycopersicum L.) rhamnogalacturonan lyase promoter.

The enzyme rhamnogalacturonan lyase (RGL) cleaves α-1,4 glycosidic bonds located between rhamnose and galacturonic acid residues in the main chain of rhamnogalacturonan-I (RG-I), a component of the plant cell wall polymer pectin. Although the mode of action of RGL is well known, its physiological functions associated with fruit biology are less understood. Here, we generated transgenic tomato plants expressing the β-glucuronidase (GUS) reporter gene under the control of a -504 bp or a -776 bp fragment of the promoter of a tomato RGL gene, Solyc11g011300.