Detection of genomic variants in BRCA1 and BRCA2 across gastric cancer patients using next generation sequencing.

Objectives: To explore the landscape of BRCA1/2 mutations in gastric cancer patients.

Methods: Next-generation sequencing (NGS), Sanger sequencing, reverse transcription quantitative polymerase chain reaction (RT-qPCR), Immunohistochemistry, The Cancer Genome Atlas (TCGA), gnomAD, and DAVID.

Results: With 95% of bases boasting a phred score surpassing 30 and a minimum coverage depth of 500X, our NGS approach ensures high-quality data acquisition.

Oxidative stress drives potent bactericidal activity of pyrazinamide against .

Pyrazinamide (PZA) is a critical component of tuberculosis first-line therapy due to its ability to kill both growing and non-replicating drug-tolerant populations of within the host. Recent evidence indicates that PZA acts through disruption of coenzyme A synthesis under conditions that promote cellular stress. In contrast to its bactericidal action , PZA shows weak bacteriostatic activity against in axenic culture.

Etiological and histomorphological studies on early chick mortality in broiler chicken in Kashmir, India.

Background: Early chick mortality (ECM) is one of the most important problems of the poultry industry that causes severe economic losses to the farmers. The chick mortality varies in different geographical locations and its etiological factor also varies.

Aim: The aim of the present work was to isolate and identify various etiological agents responsible for causing ECM in broilers, and study the overall occurrence and pathology of various disease conditions responsible for causing ECM in broilers.

Silver nanoparticle toxicity in rainbow trout: insights into physiological and molecular responses.

Metallic nanoparticles, with their large surface area to volume ratio, are increasingly important in various life fields, but they can cause varying toxic effects on fish. This study investigates the toxicological effects of silver nanoparticles (AgNPs) on rainbow trout (), focusing on hematological, biochemical, antioxidant, and histopathological changes. Fish were exposed to varying concentrations of AgNPs (0.

Toxicological impact of copper nanoparticles on rainbow trout: hematological, biochemical, antioxidant, and histopathological responses with oxidative gene expression.

Copper nanoparticles (CuNPs) are increasingly used across various industries due to their catalytic, antimicrobial, and electrical properties. However, their potential toxicity in aquatic environments, particularly to non-target organisms like fish, remains poorly understood. This study investigated the effects of CuNP exposure on rainbow trout () by comprehensively evaluating hematological, biochemical, antioxidant, molecular, and histopathological parameters.