A novel transgenic reporter of extracellular acidification in zebrafish elucidates skeletal muscle T-tubule pH regulation.

Disruption of extracellular pH and proton-sensing can profoundly impact cellular and protein functions, leading to developmental defects. To visualize changes in extracellular pH in the developing embryo, we generated a zebrafish transgenic line that ubiquitously expresses the ratiometric pH-sensitive fluorescent protein pHluorin2, tethered to the extracellular face of the plasma membrane using a glycosylphosphatidylinositol (GPI) anchor. Monitoring of pHluorin2 with ratiometric fluorescence revealed dynamic and discrete domains of extracellular acidification over the first 72 h of embryonic development.

Delivering Guideline-Concordant Care for Patients With High-Risk HPV and Normal Cytologic Findings.

Importance: As US health care systems shift to human papillomavirus (HPV)-based cervical cancer screening, more patients are receiving positive high-risk non-16/18 genotype HPV results and negative for intraepithelial lesion or malignancy (NILM) cytological findings. Risk-based management guidelines recommend 2 consecutive negative annual results to return to routine screening.

Objective: To quantify patterns of surveillance testing and associated outcomes for patients after an HPV-positive results and NILM cytologic findings.

Support groups for family members of older adults in residential aged care: A pilot feasibility study.

Objectives: There are limited mental health support services in Australia that address the well-being of family members of aged care residents. The aim of this project was to evaluate the feasibility, acceptability and preliminary effectiveness of an online program designed to support residents' families.

Methods: This one-arm mixed methods project examined uptake, attendance and retention patterns, satisfaction and experience with the service, and pre- and postoutcomes with respect to depressive and anxiety symptoms and loneliness.

A novel method for quantitative analysis of subjective experience reports: application to psychedelic visual experiences.

Introduction: Psychedelic compounds such as LSD, psilocybin, mescaline, and DMT can dramatically alter visual perception. However, the extent to which visual effects of psychedelics consistently vary for different substances is an open question. The visual effects of a given psychedelic compound can range widely both across and within individuals, so datasets with large numbers of participants and descriptions of qualitative effects are required to adequately address this question with the necessary sensitivity.