CRISPR/Cas9-mediated мultiplexed multi-allelic mutagenesis of genes located on A, B and R subgenomes of hexaploid triticale.

The first-time generation of hexaploid triticale plants harbouring variable panels of novel mutations in gene families involved in starch biosynthesis has been achieved by the subgenome-independent multiplexed CRISPR/Cas9-mediated editing.

[Transmission and exchange of genetic information during bacterial conjugation in ground-based simulations of the factors of orbital flight].

Control laboratory experiments on bacterial conjugation under simulated spaceflight conditions were performed with the use of new equipment (bioreactor RECOMB-2 and container BIOMAGNISTAT) within the RSA-NASA science program. External parameters were selected and the plan of simulation of a space experiment was verified to ensure high efficiency of the conjugative transfer of chromosomal and plasmid DNA and storage of hybrids on the ground. Genetic analysis of conjugative hybrids E.

[Virus-induced dyslipidemias as possible risk factors in the development of somatic diseases].

Experimental and clinical studies showed a number of virus infections to be accompanied by lipidemic disorders. Experimentally, dyslipidemias were found in tick-borne encephalitis (TBE) in mice, rotavirus infection in rabbits, and amyotrophic leukospongiosis in guinea pigs. The possibility of correcting the virus-induced lipidemic disorders with an antiviral drug, lincomycin, was demonstrated in TBE in mice.

[Dyslipidemia in herpetic infection].

Herpes ophthalmicus (HO) patients were examined for lipid spectrum of the serum. The tests revealed dyslipidemia (DLE) with a distinctive rise in the levels of total cholesterol (CS), alpha-CS and beta-CS. The severity of the lipidemic shifts correlated with that of the infection.

[Lipid metabolism in experimental virus infections].

The effect of herpes simplex virus (HSV) and influenza virus (IV) on lipid metabolism was studied. In conditions of acute herpetic infection of rabbits we detected typical dyslipidemia, characterized by increased contents of total cholesterol, beta-cholesterol and triglycerides in the absence of trustworthy differences in concentrations of alpha-cholesterol. The use of antiherpetic preparation furavir, on the background of infection, corrected lipid spectrum of the infected animals.