Global amyloid burden enhances network efficiency of tau propagation in the brain.

Background: Amyloid-β (Aβ) and hyperphosphorylated tau are crucial biomarkers in Alzheimer's disease (AD) pathogenesis, interacting synergistically to accelerate disease progression. While Aβ initiates cascades leading to tau hyperphosphorylation and neurofibrillary tangles, PET imaging studies suggest a sequential progression from amyloidosis to tauopathy, closely linked with neurocognitive symptoms.

Objective: To analyze the complex interactions between Aβ and tau in AD using probabilistic graphical models, assessing how regional tau accumulation is influenced by Aβ burden.

Clean and Complete Protein Digestion with an Autolysis Resistant Trypsin for Peptide Mapping.

Peptide mapping requires cleavage of proteins in a predictable fashion so that target protein-specific peptides can be reliably identified and quantified. Trypsin, a commonly used protease in this process, can also undergo self-cleavage or autolysis, thereby reducing the effectivity and even cleavage specificity at lysine and arginine residues. Here, we report highly efficient and reproducible peptide mapping of biotherapeutic monoclonal antibodies.

PodGPT: An audio-augmented large language model for research and education.

The proliferation of scientific podcasts has generated an extensive repository of audio content, rich in specialized terminology, diverse topics, and expert dialogues. Here, we introduce a computational framework designed to enhance large language models (LLMs) by leveraging this informational content from publicly accessible podcast data across science, technology, engineering, mathematics and medical (STEMM) disciplines. This dataset, comprising over 3, 700 hours of audio content, was transcribed to generate over 42 million text tokens.

Evaluation of treatment with human chorionic gonadotropin at transfer of in vitro-produced beef embryos on reproductive outcomes in lactating multiparous Jersey cows after a synchronized ovulation.

Our objective was to evaluate the effect of treatment with human chorionic gonadotropin (hCG) at the time of transfer of in vitro-produced (IVP) beef embryos on pregnancy outcomes in lactating multiparous Jersey cows. Grade 1, stage 7 (expanded blastocyst), IVP beef embryos were produced from black Angus-based dams using 3 proven high-fertility Angus sires and were frozen for direct transfer. In a preliminary experiment, lactating multiparous Jersey cows were randomized to a 2 × 2 factorial arrangement of treatments to test the main effect of recipient synchronization protocol (Double-Ovsynch; DO; n = 169 vs.