[Characteristics of cardiac growth and energy metabolism in the development of the clawed toad].

The relative content of the actomyosin complex proteins increases with respect to the total heart protein during the heart growth in Xenopus laevis. The intensity of the heart sections respiration was shown to depend on the heart weight. Constants were determined in an equation describing this dependence.

[Changes in the growth rates, respiration level and mitochondrial mass of the heart during chick embryo development].

The growth of heart during the chick embryo development is accompanied by the increase in total protein content per weight unit of the organ and in the content of the actomyosin complex proteins calculated to the total heart protein. Changes in the rate of heart respiration during development are determined, mainly, by the mass of mitochondria. The growing heart at the early developmental stages is characterized by a very high rate of doubling of the mitochondrial protein mass.

[Substrate specificity of multiple forms of glucose-6-phosphate dehydrogenase in Misgurnus fossilis loach embryos].

Studies have been made on substrate specificity of electrophoretically different forms of glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP+-1-oxidoreductase; EC 1.1.1.

[Synthesis of ribosomal proteins in early development of the loach].

The synthesis of ribosomal proteins (r-proteins) in the loach embryos was studied by the way of analysis of protein-labeled ribosomes isolated from the cytoplasm and purified from de novo synthesized polypeptide and contaminating proteins. The ribosomes at the mid-blastula and mid-gastrula stages were shown to contain no de novo synthesized proteins. The synthesis of r-proteins at the stage of organogenesis was revealed by sedimentation analysis of ribosomes in the gradient of sucrose concentrations, density analysis in the gradient of cesium chloride and analysis of the distribution of radioactivity in r-proteins separated from the labelled ribosomes by electrophoresis in polyacrilamide gel.

[Protein synthesis in loach embryos isolated from the yolk and cultivated in vitro].

Changes in protein synthesis in the loach (Misgurnus fossilis) blastoderms cultivated in vitro in the Holtfreter solution after their separation from the yolk at the early and late-blastula stages were assessed by biochemical and autoradiographic methods. The embryos incubated in vitro at the from blastula stage are characterized by the sharp activation of protein synthesis and the vegetal-animal gradient of protein synthesis, as well as in the control embryos; such embryos gastrulate and proceed to primary differentiation. On the contrary, in the embryos incubated from the early blastula stage the protein synthesis is inhibited and no regional differences in its intensity are noted; such embryos do not proceed to gastrulation.