Erratum to "characterization and diagnostic potential of hepatitis B virus nucleocapsid expressed in E. coli and P. pastoris".

The hepatitis B core antigen (HBcAg) was expressed in Escherichia coli and in Pichia pastoris. A hexa-histidine tag was introduced at the C terminus of the E. coli expressed protein allowing its purification by Ni2+-chelate affinity chromatography.

Characterization and diagnostic potential of hepatitis B virus nucleocapsid expressed in E. coli and P. pastoris.

The hepatitis B core antigen (HBcAg) was expressed in Escherichia coli and in Pichia pastoris. A hexahistidine tag was introduced at the C terminus of the E. coli expressed protein allowing its purification by Ni(2+)-chelate affinity chromatography.

Specific detection of enteroviruses in clinical samples by molecular hybridization using poliovirus subgenomic riboprobes.

Enteroviruses were specifically detected in crude clinical specimens or in cell cultures in which the viruses were amplified by dot hybridization by using poliovirus type 1-derived, subgenomic radiolabeled cRNA probes (riboprobes). The sensitivity of this test varied from 2.5 to 33%, when clinical specimens without cell culture were examined, and was about 85% in cell culture lysates.

Comparison of a non-radioactive hybridization assay for detection of hepatitis B virus DNA with the radioactive method.

Biotin and 32P-labelled HBV probes were compared in 150 sera analysed with a spot hybridization test. In 144 cases (96%) results were concordant. The non-isotopic procedure was therefore not only simple and rapid to perform, but also sensitive and specific.

[Neutralizing activity against herpes simplex in maternal milk].

Human milk could play a role in the protection of the newborn from Herpes simplex virus 2 contamination. Neutralizing activity against Herpes simplex virus 2 was measured in 94 human milk samples and in 31 sera from healthy women. A weak neutralizing activity was detected in 48 milk samples.