Publications by authors named "M Puttabyatappa"
Article Synopsis
- The cumulus-oocyte complex (COC) is important for ovulation, with its migratory abilities influenced by the expression of various proteases and their inhibitors.
- In rats, human chorionic gonadotropin (hCG) significantly boosts the expression of specific proteases and inhibitors during ovulation, which can also be replicated with forskolin and ampiregulin in cultured COCs.
- Comparative studies between rat and human COCs show differences in protease expression, suggesting that these enzymes are crucial for the migratory behavior of the COC at the time of ovulation.
Article Synopsis
- - Fetal growth restriction (FGR) is a serious pregnancy complication linked to negative outcomes like preterm birth and stillbirth, often caused by issues with placental development and nutrient supply, which can be affected by the fetus's sex.
- - The study examined changes in gene expression and miRNA associated with placental dysfunction in FGR pregnancies using tissue samples from both FGR and control pregnancies, discovering that specific genes related to angiogenesis and other factors were altered.
- - Findings indicated that placental issues in FGR pregnancies may differ based on the sex of the newborn, with notable variations in gene expression and microvessel markers, suggesting that these changes might influence how placental impairment occurs.
The placenta mediates adverse pregnancy outcomes, including preeclampsia, which is characterized by gestational hypertension and proteinuria. Placental cell type heterogeneity in preeclampsia is not well-understood and limits mechanistic interpretation of bulk gene expression measures. We generated single-cell RNA-sequencing samples for integration with existing data to create the largest deconvolution reference of 19 fetal and 8 maternal cell types from placental villous tissue (n = 9 biological replicates) at term (n = 40,494 cells).
View Article and Find Full Text PDFDevelopmental programming: Adipose depot-specific regulation of non-coding RNAs and their relation to coding RNA expression in prenatal testosterone and prenatal bisphenol-A -treated female sheep.
Mol Cell Endocrinol
March 2023
Inappropriate developmental exposure to steroids is linked to metabolic disorders. Prenatal testosterone excess or bisphenol A (BPA, an environmental estrogen mimic) leads to insulin resistance and adipocyte disruptions in female lambs. Adipocytes are key regulators of insulin sensitivity.
View Article and Find Full Text PDFGestational Bisphenol A (BPA) exposure leads to peripheral insulin resistance, and hepatic and skeletal muscle oxidative stress and lipotoxicity during adulthood in the female sheep offspring. To investigate transcriptional changes underlying the metabolic outcomes, coding and non-coding (nc) RNA in liver and muscle from 21-month-old control and prenatal BPA-treated (0.5 mg/kg/day from days 30 to 90 of gestation; Term: 147 days) female sheep were sequenced.
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