Publications by authors named "M Protasoni"
Senescent cells play a causative role in many diseases, and their elimination is a promising therapeutic strategy. Here, through a genome-wide CRISPR/Cas9 screen, we identify the gene PPIF, encoding the mitochondrial protein cyclophilin D (CypD), as a novel senolytic target. Cyclophilin D promotes the transient opening of the mitochondrial permeability transition pore (mPTP), which serves as a failsafe mechanism for calcium efflux.
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- AI in dental diagnostics is evolving, particularly in cephalometric analysis, where new open-source software helps extract important measurements from limited field of view images, reducing manual input.
- The software uses predictive algorithms to estimate missing cephalometric landmarks, testing its accuracy against actual measurements and showing promising results, although some variability remains.
- This advancement in AI could make dental diagnostics more efficient and reduce the necessity for additional X-rays, highlighting the need for further development and integration of AI in healthcare practices.
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- Scanning electron microscopy (SEM) is essential for analyzing materials at the nanoscale, but accurate sample repositioning remains a challenge that affects analysis quality.
- This study developed a LEGO®-based sample positioning system to enhance precision in identifying and aligning features during SEM sessions, showing minimal displacements and reliable results across multiple repetitions.
- The findings indicate this LEGO-based system can improve SEM analysis repeatability and accuracy, though further design optimization and application evaluations are needed for broader use in materials science research.
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- Mitochondrial oxidative phosphorylation defects are linked to various neurological and neuromuscular diseases, and primary dermal fibroblasts are commonly used to study these disorders.
- The study explored how using galactose or fructose instead of glucose in culture affects fibroblast energy metabolism, revealing that both sugars promote more oxidative processes while reducing cell growth, with galactose having a more significant impact on proliferation.
- Results indicate that while both sugars enhance mitochondrial function, fructose is better suited for studying partial oxidative phosphorylation defects due to its less drastic effect on cell growth compared to galactose.
We investigated the interfaces of the epiphyseal plate with over- and underlying bone segments using an integrated approach of histochemistry, microtomography and scanning electron microscopy (SEM) to overcome the inherent limitations of sections-based techniques. Microtomography was able to provide an unobstructed, frontal view of large expanses of the two bone surfaces facing the growth plate, while SEM observation after removal of the soft matrix granted an equally unhindered access with a higher resolution. The two interfaces appeared widely dissimilar.
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