Preclinical use of a clinically-relevant scAAV9/SUMF1 vector for the treatment of multiple sulfatase deficiency.

Background: Multiple Sulfatase Deficiency (MSD) is a rare inherited lysosomal storage disorder characterized by loss of function mutations in the SUMF1 gene that manifests as a severe pediatric neurological disease. There are no available targeted therapies for MSD.

Methods: We engineered a viral vector (AAV9/SUMF1) to deliver working copies of the SUMF1 gene and tested the vector in Sumf1 knock out mice that generally display a median lifespan of 10 days.

Grasping Post-Traumatic Stress Disorder (PTSD) from the Perspective of Psychoneuroimmunoendocrinology (PNIE): Etiopathogenic Mechanisms and Relevance for the Integrative Management.

Post-Traumatic Stress Disorder (PTSD) is a debilitating condition caused by exposure to traumatic events, affecting 5-10% of the population, with increased prevalence among women and individuals in war zones. Beyond psychological symptoms, PTSD induces significant physiological changes across systems. Psychoneuroimmunoendocrinology (PNIE) offers a framework to explore these complex interactions between the psyche and the nervous, immune, and endocrine systems.

Endothelialectomy With Trypan Blue Staining to Enhance Descemetorhexis Visualization.

Purpose: To describe a technique involving combined endothelialectomy and trypan blue staining to allow for improved visualization and Descemet membrane (DM) removal during endothelial keratoplasty.

Methods: Endothelialectomy with 2 disposable endothelial irrigating cannulas (Vortex and Sterimedix) and an irrigation-aspiration handpiece are described. Several passes over the desired area are made to ensure adequate endothelialectomy treatment.

The process map of penile prosthesis implantation in outpatient surgery in Spain: a consensus document.

Penile prosthesis (PP) implantation is feasible as an outpatient surgery. The present study describes the surgical process and establishes a consensus for improving the care circuit for outpatient PP implantation in Spain. A working group composed of a scientific committee with extensive experience in PP implantation and representatives of important scientific societies reached a consensus about the recommendations for outpatient PP implantation.

Exploring the contribution of the dopaminergic and noradrenergic systems in the antidepressant-like action of 1-(2-(4-(4-ethylphenyl)-1H-1,2,3-triazol-1-yl)phenyl)ethanone in mice.

1-(2-(4-(4-ethylphenyl)-1H-1,2,3-triazol-1-yl)phenyl)ethanone (ETAP) is a novel hybrid compound containing 1,2,3-triazole and acetophenone. It exhibits antidepressant-like effects in male mice, linked to modulation of serotonergic receptors and monoamine oxidase A (MAO-A) inhibition. This study aimed to evaluate the involvement of the dopaminergic and noradrenergic systems, as well as MAO-B activity inhibition, in the antidepressant-like effect of ETAP in male mice, and to evaluate the antidepressant-like effect of ETAP in female mice.