Influence of Klotho Protein Levels in Obesity and Sarcopenia: A Systematic Review.

The gene is recognized for its anti-aging properties. Its downregulation leads to aging-like phenotypes, whereas overexpression can extend lifespan. Klotho protein exists in three forms: α-klotho, β-klotho and γ-klotho.

Conventional PCR Versus Next Generation Sequencing for Diagnosis of FLT3, IDH and NPM1 Mutations in Acute Myeloid Leukemia: Results of the PETHEMA PCR-LMA Study.

Background/objectives: This PETHEMA PCR-LMA study aimed to evaluate whether mutations detected by NGS (VAF cut-off of ≥5%) correlate with NPM1, FLT3-ITD, FLT3-TKD, IDH1, and IDH2 mutations detected using conventional PCR (analytical sensitivity 3%) in a nationwide network of seven reference laboratories.

Methods: Between 2019 and 2021, 1685 adult AML patients with at least one centralized sample (NGS or PCR) at primary diagnosis or relapse/refractory episode were included.

Results: During this period, 1288 paired NGS/PCR samples (1094 at diagnosis, 103 at relapse and 88 at refractoriness) were analyzed.

Goodness-of-fit Tests for Categorical Models of Psychological Processes: Fixing the Occasional Failures of Asymptotic Theory.

The goodness of fit of categorical models of psychological processes is often assessed with the log-likelihood ratio statistic (), but its underlying asymptotic theory is known to have limited empirical validity. We use examples from the scenario of fitting psychometric functions to psychophysical discrimination data to show that two factors are responsible for occasional discrepancies between actual and asymptotic distributions of . One of them is the eventuality of very small expected counts, by which the number of degrees of freedom should be computed as (1) × , where is the number of response categories in the task, is the number of comparison levels, is the number of free parameters in the fitted model, and is the number of cells in the implied  ×  table in which expected counts do not exceed 0.

Outcomes and Current State of Deep Inferior Epigastric Perforator Flap Surgery in Peru and Mexico.

Background: In developing countries, breast reconstruction has multiple barriers, especially related to microsurgical procedures. Our aim was to describe the characteristics and outcomes of patients who underwent deep inferior epigastric artery perforator (DIEP) flap in 2 Latin American countries (Peru and Mexico) performed by recent postgraduate microsurgery fellows.

Methods: A retrospective study of a case series of breast cancer patients who underwent DIEP flap surgery in 5 surgery centers in 2 different countries, Peru and Mexico, was conducted.

Thermal treatment and densification of manure and biomass blends to produce stabilized soil amendments.

Land application of dairy manure is the most common practice for disposal of this waste. Agricultural fields surrounding concentrated animal feeding operations (CAFOs) often have high levels of N and P because of manure over-application. However, its low bulk density limits the amount of manure that can be profitably transported for use as fuel or fertilizer.