Detection of human papillomavirus gene sequences in cell lines derived from laryngeal tumors.

The role of Human Papillomaviruses (HPV) in laryngeal carcinomas has been studied with conflicting results. To evaluate the etiologic relationship between HPV infection and epithelial malignancy of the larynx we studied five laryngeal carcinoma cell lines obtained from patients undergoing surgery for laryngeal tumors. The paraffin embedded biopsy samples of the original tumor and different passages of the new established cell lines were investigated by PCR with consensus primers specific for HPV DNA.

Observations regarding the neurocytokaryological study of the experimental infection with herpes simplex virus type 1 and type 2 in the white mouse.

Mice distributed in groups were inoculated with the herpes virus simplex type 1 and type 2 strains in various dilutions (10(-1)-10(-7)), which had been maintained on primary human embryo cell cultures. The animals were killed and homogenates were prepared of their brains, which were used for testing the infective titre in vivo and for the isolation of nuclei, by means of the Hymer-Kuff technique. For the cytokaryological examinations, smears were prepared from the homogenate, which were stained by May-Grünwald-Giemsa and Mann methods.

Immunogenicity of inactivated vaccines prepared with parainfluenza viruses type 1 and type 3, administered experimentally by nasal or oral route.

The inactivated vaccines prepared with parainfluenza viruses type 1 and type 3, administered to mice by nasal or oral route, either as monovalent preparations in succession or as bivalent associated preparations according to the experimental models used, imparted a significant protection against the infection with the homologous active viruses. The routes of administration of vaccines, nasal and oral, which make equal demands upon the immune secretory serum and cell system, as well as the alternative of inactivated preparations for active virus vaccines are discussed.

Virological and pathomorphological aspects in experimental infections with some triassociated pneumotropic viruses in the white mouse.

Experimental infections were induced in white mice by intranasal administration of parainfluenza virus type 3, 739-2D strain, to which influenza virus A/Beijing, 353/89 (H3N2) strain, and respiratory syncytial virus, Long strain, were associated. The model was organized so as to obtain a triassociated infection, parainfluenza virus type 3 being inoculated the first and the other two viruses, in the following stages-II and III-, alternately. The infections were revealed by the presence of positive immunofluorescence reactions in the pulmonary tissue, of histological, histochemical and histoenzymatic lesions at the level of the respiratory apparatus, as well as of pathomorphological changes in other organs.

Some virological and pathomorphological aspects of the respiratory system in the experimental infection with respiratory syncytial virus associated with influenza virus, parainfluenza virus type 3 and adenovirus in the mouse.

Infections with respiratory syncytial virus Long strain, associated with influenza virus, A/Beijing 353/89 (H3N2) strain, parainfluenza virus type 3, 739-2D strain, and adenovirus type 3, were experimentally induced in white mice, causing histological, histochemical and histoenzymatic lesions at the respiratory system level, the severity of which exceeded the one observed in the controls infected with a single virus. The pathomorphological changes made up an inflammatory, predominantly infiltrative, lymphohistiocytic, then exudative and alterative picture. The severest and most frequent lesion was the diffuse interstitial, often peribronchiolovascular, bronchopneumonia, which might involve large parenchyma areas.