Hammondia hammondi cysts in cell cultures.

In an attempt to obtain the continuous development of Hammondia hammondi in vitro, culture of the parasite was performed in 3 cell lines. Although organisms were present in culture fluids of feline kidney cells (CRFK) for as long as 3 mo, continuous culture was not possible. However, for the first time, cysts of H.

A DNA fingerprinting method for individual characterization of Toxoplasma gondii strains: combination with isoenzymatic characters for determination of linkage groups.

The genetic polymorphism of Toxoplasma gondii was evaluated for 14 strains by isoenzyme and DNA analysis. The 14 strains belonged to 5 different zymodemes defined by the variable patterns of 6 enzyme systems. A restriction-fragment-length polymorphism analysis was carried out with two endonucleases (Sal I and Pst I) and two repetitive probes (TGR1E and TGR6).

Isoenzyme analysis of 35 Toxoplasma gondii isolates and the biological and epidemiological implications.

Isoenzyme analysis was conducted on the tachyzoite stage of 35 Toxoplasma gondii isolates. Fifteen enzyme systems were studied after isoelectrofocusing of tachyzoite extracts in polyacrylamide or agarose gels. Six enzyme systems showed variable electrophoretic patterns: aspartate aminotransferase (EC 2.

Isoenzyme analysis of Hammondia hammondi and Toxoplasma gondii sporozoites.

Isoenzyme analysis using isoelectrofocusing in polyacrylamide gels was used to distinguish Hammondia hammondi and Toxoplasma gondii sporozoites. Five enzyme systems were studied: aconitase (EC 4.2.