Unraveling Ceftriaxone Dosing: Free Drug Prediction, Threshold Optimization, and Model Validation.

Ceftriaxone is pivotal in treating severe infections; however, predicting unbound plasma ceftriaxone (CEF) from total ceftriaxone (CEF) remains challenging. This study aimed to (1) predict CEF from CEF, (2) determine optimal target for CEF trough concentration in plasma, (3) perform an external validation of published models, and (4) to ascertain whether the CEF dosing regimen was sufficient to achieve the therapeutic objectives. CEF predictions based on CEF were evaluated using previously published models.

Are causing recurrent cystitis just ordinary uropathogenic (UPEC) strains?

Specific determinants associated with Uropathogenic (UPEC) causing recurrent cystitis are still poorly characterized. Using strains from a previous clinical study (Vitale study, clinicaltrials.gov, identifier NCT02292160) the aims of this study were (i) to describe genomic and phenotypic traits associated with recurrence using a large collection of recurrent and paired sporadic UPEC isolates and (ii) to explore within-host genomic adaptation associated with recurrence using series of 2 to 5 sequential UPEC isolates.

The EFLM European Urinalysis Guideline 2023.

Background: The EFLM Task and Finish Group Urinalysis has updated the ECLM European Urinalysis Guidelines (2000) on urinalysis and urine bacterial culture, to improve accuracy of these examinations in European clinical laboratories, and to support diagnostic industry to develop new technologies.

Recommendations: Graded recommendations were built in the following areas.

Medical Needs And Test Requisition: Strategies of urine testing are described to patients with complicated or uncomplicated urinary tract infection (UTI), and high or low-risk to kidney disease.

Are causing recurrent cystitis just ordinary Uropathogenic (UPEC) strains?

Specific determinants associated with Uropathogenic (UPEC) causing recurrent cystitis are still poorly characterized. The aims of this study were (i) to describe genomic and phenotypic traits associated with recurrence using a large collection of recurrent and paired sporadic UPEC isolates, and (ii) to explore within-host genomic adaptation associated with recurrence using series of 2 to 5 sequential UPEC isolates. Whole genome comparative analyses between 24 recurrent cystitis isolates (RCIs) and 24 phylogenetically paired sporadic cystitis isolates (SCIs) suggested a lower prevalence of putative mobile genetic elements (MGE) in RCIs, such as plasmids and prophages.