Kinetics of the incorporation of dietary fatty acids into serum cholesteryl esters, erythrocyte membranes, and adipose tissue: an 18-month controlled study.

Tissue levels of n-3 fatty acids reflect dietary intake, but quantitative data about rate of incorporation and levels as a function of intake are scarce. We fed 58 men 0, 3, 6, or 9 g/d of fish oil for 12 months and monitored fatty acids in serum cholesteryl esters, erythrocytes, and subcutaneous fat during and after supplementation. Eicosapentaenoic acid (EPA) in cholesteryl esters plateaued after 4-8 weeks; the incorporation half-life was 4.

Dual anomeric specificity of phosphomannoisomerase assessed by 2D phase sensitive 13C EXSY NMR.

The reversible conversion between D-mannose 6-phosphate and D-fructose 6-phosphate catalyzed by yeast phosphomannoisomerase was studied by phase sensitive 2D 13C-(1H) EXSY NMR spectroscopy at 100.623 MHz, using 13C enriched substrates in the C2 position of the D-hexose 6-phosphates. The unique pair of isomerization cross-peaks observed in the 2D EXSY map correlates the 13C2 resonances of the beta-anomers of both D-[2-13C]-mannose 6-phosphate and D-[213C]-fructose 6-phosphate.

Biological markers of dietary intake, with emphasis on fatty acids.

The level of certain polyunsaturated fatty acids in body fluids or tissues can be a valid indicator of their consumption in man. In 59 housewives studied over a 2.5-year period we found a correlation of 0.

Steroidogenesis during induced oocyte maturation and ovulation in the African catfish,Clarias gariepinus.

Changes in ovarian steroidogenesis accompanying oocyte maturation and ovulation were studied in the African catfish,Clarias gariepinus. Laboratory-reared females with postvitellogenic ovaries were treated with pimozide and LHRH-analogue. The plasma gonadotropin levels were determined by means of a homologous radioimmunoassay, the condition of the ovaries was studied by histological examination of the follicles, and the steroidogenetic capacity of the ovaries was analyzed byin vitro incubation of tissue fragments for 3 h with [(3)H]-pregnenolone and [(3)H]androstenedione as precursors.