Label-free nanoscopy of cell metabolism by ultrasensitive reweighted visible stimulated Raman scattering.

Super-resolution imaging of cell metabolism is hindered by the incompatibility of small metabolites with fluorescent dyes and the limited resolution of imaging mass spectrometry. We present ultrasensitive reweighted visible stimulated Raman scattering (URV-SRS), a label-free vibrational imaging technique for multiplexed nanoscopy of intracellular metabolites. We developed a visible SRS microscope with extensive pulse chirping to improve the detection limit to ~4,000 molecules and introduced a self-supervised multi-agent denoiser to suppress non-independent noise in SRS by over 7.

The diagnostic accuracy of EyeGuide Focus testing for concussion in elite male Rugby players.

Introduction: Smooth pursuit eye movements may be affected by head impacts. The EyeGuide Focus system is a simple, portable, test of visual tracking. This study investigated the diagnostic accuracy of EyeGuide measurements for detection of concussion during elite Rugby matches.

Deep learning and genome-wide association meta-analyses of bone marrow adiposity in the UK Biobank.

Bone marrow adipose tissue is a distinct adipose subtype comprising more than 10% of fat mass in healthy humans. However, the functions and pathophysiological correlates of this tissue are unclear, and its genetic determinants remain unknown. Here, we use deep learning to measure bone marrow adiposity in the femoral head, total hip, femoral diaphysis, and spine from MRI scans of approximately 47,000 UK Biobank participants, including over 41,000 white and over 6300 non-white participants.

Repeatability, reproducibility and normative ranges for EyeGuide Focus testing system in elite male rugby players.

Objectives: The EyeGuide Focus system is a simple, portable, test of visual tracking with potential use for concussion screening. This study investigated the repeatability, reproducibility, distribution, and modifiers of EyeGuide Focus measurements in healthy elite Rugby players.

Design: Cross sectional repeated measures study and controlled pre-test post-test sub-study.

Dynamic heterogeneity in an stress response regulon mediates gene activation and antimicrobial peptide tolerance.

The bacterial stress response is an intricately regulated system that plays a critical role in cellular resistance to drug treatment. The complexity of this response is further complicated by cell-to-cell heterogeneity in the expression of bacterial stress response genes. These genes are often organized into networks comprising one or more transcriptional regulators that control expression of a suite of downstream genes.