Publications by authors named "M P Wand"
Aims: We aimed to identify mechanisms underlying the tolerance of Proteus mirabilis-a common cause of catheter associated urinary tract infection-to the clinically used biocides chlorhexidine (CHD) and octenidine (OCT).
Methods And Results: We adapted three clinical isolates to grow at concentrations of 512 µg ml-1 CHD and 128 µg ml-1 OCT. Genetic characterization and complementation studies revealed mutations inactivating the smvR repressor and increasing smvA efflux expression were associated with adaptation to both biocides.
Antimicrobial resistance (AMR) is a pandemic spread across multiple infectious disease-causing microbes. To provide a host-specific tool to study antibiotic susceptibility and resistance, here we develop Klebsiella pneumoniae cell-free gene expression (CFE) systems from laboratory and clinical isolates. Using proteomics, we identify relative differences and unique proteins for these new CFE systems in comparison to an Escherichia coli MG1655 CFE model.
View Article and Find Full Text PDFArticle Synopsis
- Analyzing high-dimensional data from molecular dynamics simulations is difficult, but this paper presents a neural network method to identify metastable states in the small peptide deca-alanine using only structural information.
- Traditional dimensionality reduction methods leverage trajectory data, which can be inefficient; instead, the authors introduce EncoderMap, an autoencoder that focuses on capturing essential conformational changes without temporal data.
- This novel approach not only simplifies data processing for studying peptides and proteins but also improves the quality of representations compared to established techniques, enhancing exploration of molecular configurations.
The development of new therapies against SARS-CoV-2 is required to extend the toolkit of intervention strategies to combat the global pandemic. In this study, hyperimmune plasma from sheep immunised with whole spike SARS-CoV-2 recombinant protein has been used to generate candidate products. In addition to purified IgG, we have refined candidate therapies by removing non-specific IgG via affinity binding along with fragmentation to eliminate the Fc region to create F(ab') fragments.
View Article and Find Full Text PDF