Linker histone H1.0 loads onto nucleosomes through multiple pathways that are facilitated by histone chaperones.

Linker histone H1 is an essential chromatin architectural protein that compacts chromatin into transcriptionally silent regions by interacting with nucleosomal and linker DNA, while rapidly exchanging . How H1 targets nucleosomes while being dynamic remains unanswered. Using a single-molecule strategy, we investigated human H1.

From conventional to cutting edge: an exploration of osteosarcoma treatments.

Osteosarcoma is a highly aggressive cancer in children and young adults that has a remarkably high mortality rate upon metastasis. Current standard treatments have remained largely unchanged for nearly five decades, focusing on a combination of chemotherapy with high-dose methotrexate, doxorubicin, and cisplatin, complemented by aggressive surgical resections. Despite this lack of change, recent advancements in medical research have spurred hope for more effective and less invasive approaches to managing osteosarcoma.

Native nucleosome-positioning elements for the investigation of nucleosome repositioning.

Nucleosome repositioning is essential for establishing nucleosome-depleted regions (NDRs) to initiate transcription. This process has been extensively studied using structural, biochemical, and single-molecule approaches, which require homogenously positioned nucleosomes. This is often achieved using the Widom 601 sequence, a highly efficient nucleosome positioning element (NPE) selected for its unusually strong binding to the H3-H4 histone tetramer.

Light quality affects chlorophyll biosynthesis and photosynthetic performance in Antarctic Chlamydomonas.

The perennially ice-covered Lake Bonney in Antarctica has been deemed a natural laboratory for studying life at the extreme. Photosynthetic algae dominate the lake food webs and are adapted to a multitude of extreme conditions including perpetual shading even at the height of the austral summer. Here we examine how the unique light environment in Lake Bonney influences the physiology of two Chlamydomonas species.