Compartment-Resolved Proteomics with Deep Extracellular Matrix Coverage.

The extracellular matrix (ECM) is a complex network of proteins that provides structural support and biochemical cues to cells within tissues. Characterizing ECM composition is critical for understanding this tissue component's roles in development, homeostasis, and disease processes. This protocol describes an integrated pipeline for profiling both cellular and ECM proteins across varied tissue types using mass spectrometry-based proteomics.

Early and high-throughput plant diagnostics: strategies for disease detection.

The rising global occurrence of plant pathogens highlights the need for a thorough reassessment of current disease detection and management schemes. To that end, we review the utility and limitations of the available sensing platforms deployed for phytodiagnostics in the field. We also discuss recent advances in the use of broad-spectrum biomarkers such as phytohormones and volatile organic compounds (VOCs), and assess the feasibility of deploying these platforms on a large scale.

A bovine model of hypoxia-induced pulmonary hypertension reveals a gradient of immune and matrisome response with a complement signature found in circulation.

Pulmonary hypertension (PH) is a progressive vascular disease characterized by vascular remodeling, stiffening, and luminal obstruction, driven by dysregulated cell proliferation, inflammation, and extracellular matrix (ECM) alterations. Despite the recognized contribution of ECM dysregulation to PH pathogenesis, the precise molecular alterations in the matrisome remain poorly understood. In this study, we employed a matrisome-focused proteomics approach to map the protein composition in a young bovine calf model of acute hypoxia-induced PH.