Publications by authors named "M Osaki"
Background Determining good candidates for head and neck cancer surgery in elderly patients requires consideration of various factors, such as overall health and social background, yet specific evaluation guidelines are lacking. The Glasgow Prognostic Score (GPS) is a marker used to assess nutritional status and prognosis in cancer patients. Objective This study aims to evaluate the association between the GPS and both the prognosis and postoperative complications in reconstructive surgery cases for head and neck cancer in patients aged 80 and over.
View Article and Find Full Text PDFChimeric antigen receptor (CAR) T cell is a promising therapy for cancer, but factors that enhance the efficacy of CAR T cell remain elusive. Here we perform a genome-wide CRISPR screening to probe genes that regulate the proliferation and survival of CAR T cells following repetitive antigen stimulations. We find that genetic ablation of CUL5, encoding a core element of the multi-protein E3 ubiquitin-protein ligase complex, cullin-RING ligase 5, enhances human CD19 CAR T cell expansion potential and effector functions, potentially via the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway.
View Article and Find Full Text PDFMicrocell-mediated chromosome transfer between non-identical human iPSCs.
Mol Ther Nucleic Acids
December 2024
Article Synopsis
- Microcell-mediated chromosome transfer (MMCT) is a new technique for manipulating chromosome numbers using human induced pluripotent stem cells (hiPSCs) to create disease models with hyperaneuploidy.
- The study highlights the efficiency of using hiPSCs as both donor and recipient cells in the chromosome transfer process, allowing for the tagging and transfer of specific chromosomes like Chr21 and ChrY using CRISPR-Cas9.
- This innovative method aims to improve the study of rare diseases and enhance our understanding of early development by integrating key chromosomal regions into hiPSCs.
Article Synopsis
- The study investigated the effects of cetylpyridinium chloride (CPC) and on-demand aqueous chlorine dioxide mouthwash on the SARS-CoV-2 viral load in mildly symptomatic COVID-19 patients.
- In a randomized clinical trial, participants were assigned to one of three groups: CPC mouthwash, chlorine dioxide mouthwash, or a placebo.
- Results showed that CPC mouthwash significantly reduced salivary viral titer at 10 minutes but had no effect at later intervals, whereas chlorine dioxide mouthwash showed no significant difference compared to the placebo at any time point.
Missing an entire chromosome or chromosome arm in normal diploid cells has a deleterious impact on cell viability, which may contribute to the development of specific birth defects. Nevertheless, the effects of chromosome loss in human cells have remained unexplored due to the lack of suitable model systems. Here, we developed an efficient, selection-free approach to generate partial monosomy in human induced pluripotent stem cells (iPSCs).
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