Dapagliflozin Improved Cardiac Function and Structure in Diabetic Patients with Preserved Ejection Fraction: Results of a Single Centre, Observational Prospective Study.

Sodium-glucose cotransporter inhibitors (SGLT2i) have demonstrated a reduction in cardiovascular events in diabetes and heart failure (HF). The mechanisms underlying this benefit are not well known and data are contradictory. The purpose of this study is to analyse the effect of dapagliflozin on cardiac structure and function in patients with normal ejection fraction.

The Loss-of-Function Mutation Leads to Enhanced α-L-Rhamnosidase Production by .

In L-rhamnose is catabolised to pyruvate and L-lactaldehyde, and the latter ultimately to L-lactate, via the non-phosphorylated pathway (LRA) encoded by the genes -, and that encodes a broad substrate range aldehyde dehydrogenase (ALDH) that also functions in ethanol utilisation. LRA pathway expression requires both the pathway-specific transcriptional activator RhaR ( is expressed constitutively) and the presence of L-rhamnose. The deletion of severely impairs growth when L-rhamnose is the sole source of carbon and in addition it abolishes the induction of genes that respond to L-rhamnose/RhaR, indicating that an intermediate of the LRA pathway is the physiological inducer likely required to activate RhaR.

-Mediated Transformation of NHEJ Mutant Conidia: An Efficient Tool for Targeted Gene Recombination Using Selectable Nutritional Markers.

Protoplast transformation for the introduction of recombinant DNA into is technically demanding and dependant on the availability and batch variability of commercial enzyme preparations. Given the success of -mediated transformation (ATMT) in diverse pathogenic fungi, we have adapted this method to facilitate transformation of . Using suitably engineered binary vectors, gene-targeted ATMT of non-homologous end-joining (NHEJ) mutant conidia has been carried out for the first time by complementation of a nutritional requirement (uridine/uracil auxotrophy).