Sequential window acquisition of all theoretical mass spectra (SWATH-MS) as an emerging proteomics approach for the discovery of dark-cutting beef biomarkers.

Recent advances in "omics" technologies have enabled the identification of new beef quality biomarkers and have also allowed for the early detection of quality defects such as dark-cutting beef, also known as DFD (dark, firm, and dry) beef. However, most of the studies conducted were carried out on a small number of animals and mostly applied gel-based proteomics. The present study proposes for the first time a Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS) proteomics approach to characterize and comprehensively quantify the post-mortem muscle proteome of DFD (pH ≥ 6.

-Pincers in Platinum Bimetallic Complexes: Influence of the Pincer and Bridging Ligands on the Metal-Metal Bond and the Photophysical Properties.

Precursors PtCl{κ--[py-CHMe-py]} (), PtCl{κ--[py-O-CH-O-py]} (), Pt(OH){κ--[py-CHMe-py]} (), and Pt(OH){κ--[py-O-CH-O-py]} () were used to prepare d-platinum bimetallic complexes. Precursors and react with AgBF and 7-azaindole (H) to give [Pt{κ--[py-CHMe-py]}{κ--[H]}]BF () and [Pt{κ--[py-O-CH-O-py]}{κ--[H]}]BF () and and with indolo[2,3-]indole (H) to generate Pt{κ--[H]}{κ--[py-CHMe-py]} () and Pt{κ--[H]}{κ--[py-O-CH-O-py]} (). Subsequent addition of and to - affords bimetallic derivatives [{Pt[κ--(py-CHMe-py)]}{μ--[]}]BF (), [{Pt[κ--(py-O-CH-O-py)]}{μ--[]}]BF (), and {Pt[κ--(py-CHMe-py)]}{μ--[]} ().

Exploring the miRNAs Profile in Dark-Cutting Beef.

In an animal production system, different stressors may cause the depletion of muscle glycogen stores, resulting in an elevated pH at 24 h (pH), which leads to cell metabolism alterations that affect the conversion of muscle into meat, causing meat quality defects, such as dark-cutting beef, also known as dark, firm, and dry (DFD) beef. This process may involve the alteration of small non-coding RNAs (miRNAs), which play critical regulatory roles in cellular processes. Here, we determined whether differential miRNA expression in the muscle from the Asturiana de los Valles breed at 24 h could serve as an early indicator of beef quality defects.

Muscle Hypertrophy Is Linked to Changes in the Oxidative and Proteolytic Systems during Early Tenderization of the Spanish Breed "Asturiana de los Valles".

For fresh meat consumers, eating satisfaction is of utmost importance and tenderness is one of the most important characteristics in this regard. Our study examined beef of different animal biotypes of the autochthonous breed "Asturiana de los Valles" (AV) to determine if early postmortem oxidative and proteolytic processes may influence the final tenderness of the product. This meat-specialized breed shows different biotypes depending on the frequency of a myostatin mutation "" that induces double-muscling or muscular hypertrophy (/, /+, +/+).

Two Synthetic Tools to Deepen the Understanding of the Influence of Stereochemistry on the Properties of Iridium(III) Heteroleptic Emitters.

Two complementary procedures are presented to prepare -pyridyl-iridium(III) emitters of the class [++] with two orthometalated ligands of the 2-phenylpyridine type () and a third ligand (). They allowed to obtain four emitters of this class and to compare their properties with those of the -pyridyl isomers. The finding starts from IrH(PPr), which reacts with 2-(-tolyl)pyridine to give -[Ir{κ--[CMeH-py]}] with an almost quantitative yield.