Design of experiments-based high-throughput strategy for development and optimization of efficient cell disruption protocols.

Efficient and reproducible cell lysis is a crucial step during downstream processing of intracellular products. The composition of an optimal lysis buffer should be chosen depending on the organism, its growth status, the applied detection methods, and even the target molecule. Especially for high-throughput applications, where sample volumes are limited, the adaptation of a lysis buffer to the specific campaign is an urgent need.

Model based optimization of the intermittent aeration profile for SBRs under partial nitrification.

In this paper, a fast and accurate optimization framework is proposed to compute optimal aeration policies in SBR processes under partial nitrification. The optimization framework aims to determine an optimal intermittent aeration profile which minimizes both the operation time of the SBR cycle and the energy required for aeration. Special consideration is given to the fact that the results not only need to be accurate but also to converge within a short time.