Publications by authors named "M Neefjes"
Article Synopsis
- The study aimed to differentiate osteoarthritis (OA) patients by analyzing their serum-induced cellular signaling patterns, using samples from knee OA patients, hand OA patients, and healthy controls.
- Results showed significant differences in cellular pathway activity based on the type of OA, with hand OA serum triggering higher MAPK-related AP1 activity, while knee OA serum affected other pathways related to ELK1-SRF, STAT1-STAT2, and SOX9.
- The findings suggest that the underlying mechanisms of OA differ between hand and knee OA, potentially paving the way for more targeted treatments based on specific OA endotypes.
Osteoarthritis (OA) is the most prevalent joint disease, and it is characterized by cartilage degeneration, synovitis, and bone sclerosis, resulting in swelling, stiffness, and joint pain. TAM receptors (Tyro3, Axl, and Mer) play an important role in regulating immune responses, clearing apoptotic cells, and promoting tissue repair. Here, we investigated the anti-inflammatory effects of a TAM receptor ligand, i.
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- The research aimed to compare the chondrocyte signaling profiles between non-osteoarthritic and end-stage osteoarthritic knee synovial fluid to understand their effects on cartilage cells.
- Protein profiling techniques were employed to analyze the different signaling patterns and their consequences on chondrocyte behavior.
- Findings revealed that osteoarthritic synovial fluid has more inflammatory cytokines and growth factors, leading to altered signaling that promotes abnormal chondrocyte behavior and contributes to cartilage degeneration.
During osteoarthritis (OA), hypertrophy-like chondrocytes contribute to the disease process. TGF-β's signaling pathways can contribute to a hypertrophy(-like) phenotype in chondrocytes, especially at high doses of TGF-β. In this study, we examine which transcription factors (TFs) are activated and involved in TGF-β-dependent induction of a hypertrophy-like phenotype in human OA chondrocytes.
View Article and Find Full Text PDFObjectives: Alterations in the composition of synovial fluid have been associated with adverse effects on cartilage integrity and function. Here, we examined the phenotypic and proliferative behavior of human articular chondrocytes when cultured in vitro for 13 days with synovial fluid derived from end-stage osteoarthritis patients.
Materials And Methods: Chondrocyte proliferation and phenotypical changes induced by osteoarthritic synovial fluid were analyzed using DNA staining, RT-qPCR, immunostainings, and immunoblotting.