PFGE and PCR/RFLP typing of Campylobacter jejuni strains from poultry.

1. Pulsed-field gel electrophoresis (PFGE) and PCR-restriction fragment length polymorphisms of the flagellin gene (fla-RFLP) were used to analyse 92 poultry and 110 human strains of Campylobacter jejuni. 2.

PCR/restriction fragment length polymorphism (RFLP) typing of human and poultry Campylobacter jejuni strains.

Aims: The PCR/RFLP typing of 156 isolates Campylobacter jejuni originating from poultry and humans was performed (101 human and 55 poultry strains).

Methods And Results: On the basis of restrictive digest, six types were identified with AfaI, seven types with MboI and five types with HaeIII. With a combination of these three enzymes, 22 types were found.