Ligand interaction landscape of transcription factors and essential enzymes in E. coli.

Knowledge of protein-metabolite interactions can enhance mechanistic understanding and chemical probing of biochemical processes, but the discovery of endogenous ligands remains challenging. Here, we combined rapid affinity purification with precision mass spectrometry and high-resolution molecular docking to precisely map the physical associations of 296 chemically diverse small-molecule metabolite ligands with 69 distinct essential enzymes and 45 transcription factors in the gram-negative bacterium Escherichia coli. We then conducted systematic metabolic pathway integration, pan-microbial evolutionary projections, and independent in-depth biophysical characterization experiments to define the functional significance of ligand interfaces.

Pharmacological, computational, and mechanistic insights into triptolide's role in targeting drug-resistant cancers.

As a promising candidate for tackling drug-resistant cancers, triptolide, a diterpenoid derived from the Chinese medicinal plant Tripterygium wilfordii, has been developed. This review summarizes potential antitumor activities, including the suppression of RNA polymerase II, the suppression of heat shock proteins (HSP70 and HSP90), and the blockade of NF-kB signalling. Triptolide is the first known compound to target cancer cells specifically but spare normal cells, and it has success in treating cancers that are difficult to treat, including pancreatic, breast, and lung cancers.

Biosynthesis, characterization, and multifaceted applications of Elytraria acaulis synthesized silver and gold nanoparticles: Anticancer, antibacterial, larvicidal, and photocatalytic activities.

Green synthesis of metal nanoparticles using plant extracts has emerged as an eco-friendly alternative to conventional methods, offering potential applications in biomedicine and environmental remediation. This study demonstrates the successful biosynthesis of silver nanoparticles (SNPs) and gold nanoparticles (GNPs) using Euphorbia acaulis leaf extract as a reducing and capping agent. The nanoparticles were thoroughly characterized using UV-Vis spectroscopy, HR-SEM, EDX, TEM, AFM, XRD, and FTIR analyses, confirming their successful synthesis and revealing their predominantly spherical morphology with sizes ranging from 1 to 100 nm.