Publications by authors named "M Nadji"
Objectives: Frozen section (FS) is a technique widely used intraoperatively to render a preliminary histopathologic diagnosis, allowing for immediate decisions at the time of surgery. We aimed to investigate potential variations in tissue antigenicity induced by rapid freezing in a variety of gynecologic tumor samples.
Methods: A total of 177 FS and 177 non-frozen section (NFS) tissue slides were tested using a panel of immunostains commonly used in gynecologic pathology, including hormone receptors (estrogen receptor, progesterone receptor), HER2, mismatch repair proteins (MSH6, PMS2), programmed cell death 1 ligand 1 (PD-L1), p53, napsin A, and ɑ-methylacyl coenzyme-A racemase.
Article Synopsis
- - The Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) suggests that atypia of undetermined significance (AUS) should not exceed 10%, but some recent findings indicate that it might often be overused, especially when molecular testing results are low.
- - A study analyzed the cytology cases over 4.5 years, examining AUS rates and positive call rates for different cytopathologists, revealing a wide range of AUS rates (from 9.9% to 36.8%) and an overall positive call rate of 24%.
- - The findings suggest that monitoring AUS rates alongside positive call rates can provide a valuable way to evaluate and enhance the performance of cytology labs and individual cytopath
Casein Protein Block to Optimize Immunohistochemistry Detection of Surface Immunoglobulin Heavy Chain Expression in Lymphoid Cells.
Appl Immunohistochem Mol Morphol
February 2023
We describe our recent experience of studying expression of immunoglobulin (Ig) heavy chain (IgG, IgM, and IgA) in lymphoid cells comprising a research set of formalin-fixed, paraffin-embedded human diffuse large B-cell lymphoma samples. We found that using typical clinical automated immunohistochemistry protocols and usual buffers as blocking agents, the extent of undesirable staining was extreme and impaired our ability to interpret heavy chain Ig expression by individual lymphoid cells. We were not able to optimize this with serial dilutions in antibody concentration or time of primary antibody exposure.
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