Evaluation of antioxidant properties of root bark of Hemidesmus indicus R. Br. (Anantmul).

Hemidesmus indicus R. Br. (Asclepiadaceae) is a well known drug in Ayurveda system of medicine.

HPTLC method for the estimation of alkaloids of Cinchona officinalis stem bark and its marketed formulations.

We report a sensitive method for the estimation of quinine (Qn), cinchonine (Cn), and cinchonidine (Cnd) and a new method based on fluorescence enhancement and detection and quantification of quinidine (Qnd) from Cinchona stem bark and its formulations, using HPTLC. Standard solutions of Qn, Qnd, Cn, and Cnd were applied on precoated HPTLC plates and developed with chloroform/diethylamine (9.6:1.

Sensitive high-performance thin-layer chromatographic method for the estimation of diospyrin, a tumour inhibitory agent from the stem bark of Diospyros montana Roxb.

Diospyrin, a tumour inhibitory agent from the stem bark of Diospyros montana was isolated and characterised. A sensitive high-performance thin-layer chromatographic (HPTLC) method was developed for the estimation of diospyrin. The method was validated for precision (intra- and inter-day), repeatability and accuracy.

Estimation of individual sennosides in plant materials and marketed formulations by an HPTLC method.

Senna is a well-known drug, used in the Ayurvedic and Allopathic systems of medicine, and is a treatment for constipation. The purgative action of senna and its formulations is due to the presence of sennosides A and B. An HPTLC method has been developed for the determination of individual sennosides (A, B, C, D) without any derivatization in marketed formulations (three tablet formulations, two granule formulations and one liquid formulation) and plant materials (senna leaf and pod).

A rapid method for isolation of andrographolide from andrographis paniculata nees (kalmegh).

A simple and rapid method for isolation of andrographolide from the leaves of Andrographis paniculata is reported. This involves extraction of the leaf powder by cold maceration in a 1:1 mixture of dichloromethane and methanol and isolation of andrographolide directly from the resulting extract by recrystallisation. The identity of the compound was confirmed through IR, UV, mass and melting point, and co-chromatography with a reference standard on TLC.