[Morphological features of jaw osteonecroses in injectable drug abuse].

Objective: To determine the characteristic features in the structure of jaw bone sections in patients with jaw osteonecrosis caused by the use of homemade methamphetamine (HM) synthesized by the Nagai method.

Material And Methods: The investigation material was jaw sequesters and resected sections in 60 patients, which had been taken during sanitizing surgery for chronic odontogenic osteomyelitis (Group 1, =30, a comparison group) and jaw osteonecrosis (Group 2, =30). All the patients in Group 2 confirmed that they had been using HM for at least 2 years.

[Epigenetic markers of early diagnostics of prostate cancer.].

The last 25 years, the prostate specific antigen (PSA) of the serum is used to diagnose prostate cancer. The experience of applying the PSA test showed its inconsistency as a diagnostic marker due to low cancerspecificity. Along with this, the next wave of biomarkers of prostate cancer appeared, which may supplement or, in due course, replace PSA due to higher sensitivity and cancerspecificity.

[THE EVOLUTION OF MARKERS OF PROSTATE CANCER].

The implementation of biochemical laboratory tests in oncology practice increased exponentially during last decades and continues to be in progress nowadays. The application of modern molecular genetic technologies permits using diagnostic systems with greater diagnostic sensitivity and specificity. The new tests are actively implemented permitting to diagnose physical presence of tumor systemic manifestations of malignant neoplasm (cachexia, pyrexia), paraneoplastic syndromes and also to detect tumor markers.

[Genome-wide analysis of DNA methylation in prostate cancer using the technology of Infinium HumanMethylation450 BeadChip (HM450)].

Using the technology of DNA chips Infinium HumanMethylation 450 BeadChip it was analyzed quantitative DNA methylation status in 12 paired samples of prostate adenocarcinoma, and morphologically altered tissues. Analysis of differentially methylated regions of the genome showed an association with abnormal status for 21610 and 3852 hypomethylated hyper-methylated CpG sites. Dominance in the cancer genome hypermethylated sites and their predominant localization in the regulatory regions of genes indicate their possible role in the implementation of mechanisms of gene suppression in the pathogenesis of prostate cancer (PCa).

[Decalcification in histology laboratory techniques].

The paper describes a decalcification procedure that may be used in any histology laboratory handling bone samples for general and special purposes. It discusses the advantages and disadvantages of the major types of decalcifying agents, the principles of their choice for different types of objects, and the methods for determining the endpoint of decalcification.