Publications by authors named "M N Cortinas"
Human respiratory and enteric viruses are responsible for substantial morbidity and mortality worldwide. Wastewater-based epidemiology utilizing next-generation sequencing serves as an effective tool for monitoring viral circulation dynamics at the community level. However, these complex environmental samples are often laden with other microorganisms and host genomic material, which can hinder the sensitivity of viral detection.
View Article and Find Full Text PDFThis study details a genomics-based approach for the early detection of mosquito-borne pathogens, marked by Uruguay's first ever complete genomic sequencing of Dengue Virus type I genotypes I and V. This pioneering effort has facilitated the prompt identification of these genotypes within the country, enabling Uruguayan public health authorities to develop timely and effective response strategies. Further integrated into this approach is a climate-driven suitability measure, closely associated with Dengue case reports and indicative of the local climate's role in the virus's transmission in the country within the changing climate context.
View Article and Find Full Text PDFWe report the genomic analysis from early equine cases of the Western equine encephalitis virus outbreak during 2023-2024 in Uruguay. Sequences are related to a viral isolate from an outbreak in 1958 in Argentina. A viral origin from South America or continuous enzootic circulation with infrequent spillover is possible.
View Article and Find Full Text PDFArticle Synopsis
- - Uruguay reported its first outbreak of the Chikungunya virus in 2023, which placed a heavy strain on the healthcare system.
- - Researchers analyzed data from 30 new whole genomes to understand how the virus is transmitting locally and the ecological factors influencing its spread.
- - The study aimed to provide quick insights into the outbreak to help manage and control its impact within the country.
Article Synopsis
- The rising spread of arboviruses and increasing infection rates emphasize the urgent need for improved methods to rapidly and accurately detect these pathogens.
- This study focuses on combining nanopore sequencing with reverse transcription polymerase chain reaction (RT-PCR) to create a multiplex RT-PCR protocol.
- The new protocol allows the simultaneous detection of multiple viruses, including Chikungunya, dengue, Zika, yellow fever, and West Nile, enhancing our ability to differentiate between arbovirus strains effectively.