The response of spleen dendritic cell-enriched population to bacterial and allogeneic antigens.

The dendritic cells (DC) play crucial role in initiation and modulation of immune response especially innate immune response. We investigated the influence bacterial (E. coli and S.

Human skin preserved long-term in anhydric pulverized sodium chloride retains cell molecular structure and resumes function after transplantation.

Background: Human skin is needed to cover large areas of the body lost through burns, trauma, and extensive maxillofacial surgery. Contemporary methods of skin storage are limited by the period of preservation to a few days. Our previous findings showed that fixation and storage of human skin in anhydric sodium chloride at room temperature for weeks or months preserves its morphological and molecular structure.

Human skin preserved in anhydric sodium chloride for months can be successfully transplanted.

Human skin can be preserved in pulverized sodium chloride dehydrated at 240C for 2 hours at room temperature for periods of weeks or months and successfully transplanted to scid mouse, retaining its normal morphological structure. Fragment of skin of a size of 10 x 10 x 6 mm were harvested during elective vascular and orthopaedic surgery of lower limbs, dried of blood and placed in anhydric sodium chloride powder in tight sealed containers. Prior to transplantation to scid mice, the specimens were desalinated and rehydrated.

Human keratinocyte stem cells survive for months in sodium chloride and can be successfully transplanted.

Human skin fragments can be preserved in anhydric sodium chloride at room temperature for periods of weeks or months and successfully transplanted, retaining normal morphological structure. Skin fragments of 10 x 10 x 6 mm were harvested during elective vascular and orthopedic surgery of lower limbs, dried of blood, and placed in anhydric sodium chloride powder in tightly sealed containers. Prior to transplantation to scid mice, the specimens were desalinated and rehydrated.

The influence of intraperitoneal transplantation of free and encapsulated Langerhans islets on the second set phenomenon.

To protect the allografts or xenografts against transplant rejection special semipermeable membranes are applied. So far, there are only a few studies on the influence of an immunoisolated graft on the recipient immune system. Therefore, the possibility that an intraperitoneally grafted alginate/poly L-lysine/alginate (APA) coated pancreatic islets graft can effectively sensitize the recipient and provoke second set phenomenon was studied.