Publications by authors named "M Monot"
Article Synopsis
- Defenses against oxidants, particularly superoxide scavenging enzymes (SOSEs), are vital for the survival of pathogens, especially aerobes, but much of the existing knowledge is based on bacteria that have these enzymes.
- This study explores the evolution of a pathogen lacking SOSEs and how it develops alternative mechanisms to handle superoxide stress, showing that despite losing superoxide dismutase (SOD), it can still adapt effectively.
- The research highlights the significance of pathways related to cysteine and leucine biosynthesis in combating superoxide and suggests that cysteine oxidation plays a central role in handling superoxide toxicity, challenging traditional views on how bacteria adapt to oxygen-related stress.
In the early COVID-19 pandemic with urgent need for countermeasures, we aimed at developing a replicating viral vaccine using the highly efficacious measles vaccine as vector, a promising technology with prior clinical proof of concept. Building on our successful pre-clinical development of a measles virus (MV)-based vaccine candidate against the related SARS-CoV, we evaluated several recombinant MV expressing codon-optimized SARS-CoV-2 spike glycoprotein. Candidate V591 expressing a prefusion-stabilized spike through introduction of two proline residues in HR1 hinge loop, together with deleted S1/S2 furin cleavage site and additional inactivation of the endoplasmic reticulum retrieval signal, was the most potent in eliciting neutralizing antibodies in mice.
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- Intratumoral bacteria, specifically Colibactin-producing (CoPEC) strains, are linked to tumor heterogeneity and cancer recurrence by creating a low-immunity environment in right-sided colorectal tumors.
- These bacteria foster lipid accumulation in cancer cells, which helps them survive and resist chemotherapy, correlating with worse survival rates in advanced-stage colorectal cancer patients.
- Targeting the metabolic changes induced by CoPEC with specific inhibitors has shown potential in restoring chemotherapy sensitivity, suggesting a new approach to improve treatment outcomes for patients colonized by these bacteria.
Investigations of cellular responses to viral infection are commonly performed on mixed populations of infected and uninfected cells or using single-cell RNA sequencing, leading to inaccurate and low-resolution gene expression interpretations. Here, we performed deep polyA+ transcriptome analyses and novel RNA profiling of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infected lung epithelial cells, sorted based on the expression of the viral spike (S) protein. Infection caused a massive reduction in mRNAs and long non-coding RNAs (lncRNAs), including transcripts coding for antiviral factors, such as interferons (IFNs).
View Article and Find Full Text PDFClostridioides difficile, a Gram-positive spore-forming anaerobic bacterium, has rapidly emerged as the leading cause of nosocomial diarrhoea in hospitals. The availability of large numbers of genome sequences, mainly due to the use of next-generation sequencing methods, has undoubtedly shown their immense advantages in the determination of C. difficile population structure.
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