Development of single mouse blastomeres enlarged to zygote size in conditions of nucleo-cytoplasmic synchrony.

The following blastomeres were enlarged to the size of the zygote by one, two or three rounds of blastomere enucleation and electrofusion: (1) from the 2-cell stage (referred to as 2/1 embryos), (2) from the 4-cell stage (referred to as 4/1 embryos), (3) from the 8-cell stage (referred to as 8/1 embryos). Such single enlarged blastomeres developed into blastocysts in vivo in 55.5% (2/1), 28% (4/1) and 6.

Immune monitoring after renal transplantation. II. Post-transplant disturbances in the CD3 complex expression may be caused by immunosuppression.

The in vitro effect of major immunosuppressive agents on the CD3-TCR complex expression on T cells was studied. Cyclosporine and azathioprine caused a marked diminution of alpha/beta, but not gamma/delta and CD3 chains detectability. Prednisolone effect was less manifested.

Immune monitoring after renal transplantation. I. HLA-D expression on T cells from renal allograft recipients.

The expression of class II antigens (HLA-DR, DP and DQ) on resting and PHA-activated T cells from renal allograft recipients was studied. A tendency for increase in DR+/DQ+ T cells was noted in azathioprine and cyclosporine-treated recipients undergoing rejection. Low inducible HLA-D expression (especially DP) was associated with CMV infection.

Normal B cell compartment in chronic lymphatic leukemia.

In contrast to normal B lymphocytes, CLL cells express CD5 antigen believed to be a T cell marker. We have assessed normal and malignant B cell compartments in CLL patients with the aid of two-colour immunofluorescence techniques (CD5-TRITC, DR-FITC). No clear correlation was found between progression of disease and parameters studied.

Rejection-associated upregulation of the alpha/beta-T cell receptor expression on T cells of renal allograft recipients.

Peripheral T lymphocytes of renal allograft recipients were phenotyped for their expression of the CD3-T cell receptor (TCR) complex. The majority of T cells from patients with stable graft function had normal CD3 but diminished TCR alpha/beta heterodimer expression, while TCR gamma/delta + T cells were increased in some cases. Acute rejection was associated with an increase in TCR alpha/beta + T cells to normal levels.