Reversibility of endurance training-induced changes on glucocorticoid sensitivity of monocytes by an acute exercise.

Objective: The aim of this study was to address the effect of endurance training on monocyte glucocorticoid sensitivity in vitro.

Methods: For this purpose, in vitro dexamethasone inhibition of lipopolysaccharide (LPS)-induced interleukin-6 (IL-6) secretion in cultures of peripheral monocytes was compared in 6 untrained subjects (UT) and in 6 endurance-trained (ET) men at 0800 h, 24 h after the end of the last session of exercise (ET men). Moreover, to test the plasticity of these monocytes glucocorticoid sensitivity in ET men, the effect of an acute bout of exercise was further studied 2 h after the end of a 2-h run at 65-75% VO2max (1200 h) and compared to the results of UT after 4 h of rest.

[Cross-reactivity between cow's milk and goat's milk].

Tolerance of LC may be explained by a great intra and interspecific genetic diversity that leads to a great variety of proteins. In contrast, industrial treatment (UHT sterilisation) introduces configural modifications that may provoke a very great structural homology between the proteins of the two milks. Casein is in high concentration in both milks and shows some homology of the AA sequences between both and it makes a common allergen for a cross- reaction.

[Histamine liberation in vitro or histamine release (HR). Study of the threshold of positivity in allergy to hymenopteran venoms].

The technique of histamine release in vitro (HR) is only usable in diagnosis of hymenoptera venom allergy. The allergen extracts that are supplied for clinical (skin tests) or biological diagnosis contain significant amounts of histamine, falsify the results, especially HR, and reduce significantly the correlation between HR and other biological or cutaneous tests. Dialysis of the allergen extracts significantly improves the sensitivity and specificity of the technique and lowers the threshold of positivity of HR from 30% to 12%.

[Histamine liberation (or histamine release HR): study of the positivity threshold in hymenoptera venom allergy].

Dialysis of Hymenoptera venoms for measurement of HR by the radioimmunoassay technique (RIA, Immunotech) lowers the threshold of positivity of the HR titre from 30% to 12%. This modification of dialysis of the venoms significantly improves the sensitivity and specificity of the technique.