Simple method for the determination of anthelmintic drugs in milk intended for human consumption using liquid chromatography-tandem mass spectrometry.

Background: Helminth infections in animals to be consumed by humans are an important medical and public health problem. Pharmaceutical research has focused on developing new anthelmintic drugs for parasite control in these animals. However, the incorrect use of anthelmintics can leave residues in animal products intended for human consumption.

Analysis of Isoflavones in Foods.

In recent years the nutritional and bioactive properties of foods are being intensively investigated with a view to control, in addition to food quality, their possible influence on human health. Because of this, there is a growing demand for rapid, selective, sensitive, and validated methods for analysis and quantification. Bioactive plant compounds include those with weak estrogenic activity (phytoestrogens), among which are the isoflavones.

Determination of tocopherols and sitosterols in seeds and nuts by QuEChERS-liquid chromatography.

In the present work a simple, reliable and affordable sample treatment method for the simultaneous analysis of tocopherols and free phytosterols in nuts was developed. Analyte extraction was carried out using the QuEChERS methodology and analyte separation and detection were accomplished using HPLC-DAD. The use of this methodology for the extraction of natural occurring substances provides advantages such as speed, simplicity and ease of use.

Comparative study of the methodology used in the extraction of isoflavones from legumes applying a modified QuEChERS approach.

Introduction: Isoflavones are phytochemicals of great interest because of their association with a large variety of positive effects on human health. The major sources of isoflavones in the diet are plants of the Leguminosae family, especially soybeans, although many other legumes more widely consumed in the Mediterranean diet have also been reported to contain these compounds. In previous work we extracted isoflavones from samples using a modified QuEChERS (Quick Easy Cheap Effective Rugged Safe) methodology.

A modified QuEChERS method as sample treatment before the determination of isoflavones in foods by ultra-performance liquid chromatography-triple quadrupole mass spectrometry.

This paper reports the development of an analytical method for the determination of isoflavones in legumes using LC-MS/MS. A modified approach of the QuEChERS methodology was used to extract the analytes from the food samples. The proposed method includes a two-step extraction process and allows the determination of isoflavones in pulses without the need of a clean-up step.