Exercise-induced bronchoconstriction in children: Delphi study and consensus document about definition and epidemiology, diagnostic work-up, treatment, and follow-up.

Background: Exercise-induced bronchoconstriction (EIB) is common in children with asthma but can be present also in children without asthma, especially athletes. Differential diagnosis includes several conditions such as exercise-induced laryngeal obstruction (EILO), cardiac disease, or physical deconditioning. Detailed medical history, clinical examination and specific tests are mandatory to exclude alternative diagnoses.

Spatially variable organic-matter-driven clogging in a stormwater infiltration pond: Isotopic, microbiological and hydrogeological evidence.

Stormwater infiltration ponds (SIPs) are nature-based solutions which tend to decrease their infiltration capacity over time due to pore clogging. Organic matter (OM) is a well-known clogging driver, but how OM affects the physical and biochemical processes in a SIP remains largely unknown. An analysis encompassing soil organic carbon (SOC) stable isotopes, extracellular polymeric substances (EPS) of biofilms, DNA-based identification of microbiological communities and hydrogeological tests was carried out to elucidate the main clogging mechanisms in a large SIP in Italy.

Gating residues govern ligand unbinding kinetics from the buried cavity in HIF-2α PAS-B.

While transcription factors have been generally perceived as "undruggable," an exception is the HIF-2 hypoxia-inducible transcription factor, which contains an internal cavity that is sufficiently large to accommodate a range of small-molecules, including the therapeutically used inhibitor belzutifan. Given the relatively long ligand residence times of these small molecules and the lack of any experimentally observed pathway connecting the cavity to solvent, there has been great interest in understanding how these drug ligands exit the buried receptor cavity. Here, we focus on the relevant PAS-B domain of hypoxia-inducible factor 2α (HIF-2α) and examine how one such small molecule (THS-017) exits from the buried cavity within this domain on the seconds-timescale using atomistic simulations and ZZ-exchange NMR.