Amino acid analysis by high-performance liquid chromatography with methanesulfonic acid hydrolysis and 9-fluorenylmethylchloroformate derivatization.

Experiments were undertaken to verify a method for complete amino acid analysis of plant and animal tissues and waste products from a single hydrolysis and high-performance liquid chromatographic run. Using methanesulfonic acid, hydrolysis of cytochrome c at 115 degrees C for 22 h yielded recoveries equal to or higher than hydrolysis at 115 degrees C for 70 h or at 150 degrees C for 22 h. Triple evacuation of the hydrolysis tube alternated with nitrogen flush gave recovery improvements over single evacuation.

A form of rabbit liver cytochrome P-450 that catalyzes the 7 alpha-hydroxylation of cholesterol.

A form of cytochrome P-450 which comigrates with cytochrome P-450LM4 (molecular weight, 55 000) on SDS-polyacrylamide gel was purified from liver microsomes of cholestyramine-treated rabbits. This form of cytochrome P-450 catalyzed the 7 alpha-hydroxylation of cholesterol with an activity of 37.5 pmol/min per nmol cytochrome P-450 in the reconstituted enzyme system containing cytochrome P-450 and NADPH-cytochrome P-450 reductase.

Dry matter, energy and nitrogen conversion by Lepidoptera and Hymenoptera larvae fed leaves of black cherry.

Dry matter, energy and nitrogen budgets of the form: ingestion=growth+feces+respiration, were determined for larvae of 34 species of Hymenoptera and Lepidoptera collected from and fed leaves of black cherry (Prunus serotina). The mean growth efficiencies based on energy were: gross (100 growth/ingestion)= 17±4, and net (100 growth/(ingestion-feces))=44±8. The mean nitrogen conversion efficiency was 42±10%.