Hyperplex PCR enables highly multiplexed analysis of point mutations in wastewater: Long-term SARS-CoV-2 variant surveillance in Sweden as a case study.

Wastewater-based surveillance (WBS) allows the analysis of pathogens, chemicals or other biomarkers in wastewater to derive unbiased epidemiological information at population scale. After re-gaining attention during the SARS-CoV-2 pandemic, the field holds promise as a surveillance and early warning system by tracking emerging pathogens with pandemic potential. Expanding the current toolbox of analytical techniques for wastewater analysis, we explored the use of Hyperplex PCR (hpPCR) to analyse SARS-CoV-2 mutations in wastewater samples collected weekly in up to 22 sites across Sweden between October 2022 and December 2023.

In vivo simultaneous proton resonance frequency shift thermometry and single reference variable flip angle T measurements.

Purpose: The single reference variable flip angle sequence with a multi-echo stack of stars acquisition (SR-VFA-SoS) simultaneously measures temperature change using proton resonance frequency (PRF) shift and T-based thermometry methods. This work evaluates SR-VFA-SoS thermometry in MR-guided focused ultrasound in an in vivo rabbit model.

Methods: Simultaneous PRF shift thermometry and T-based thermometry were obtained in a New Zealand white rabbit model (n = 7) during MR-guided focused ultrasound surgery using the SR-VFA-SoS sequence at 3 T.

Ferric carboxymaltose in reducing blood transfusions and infections after cardiac surgery.

Objective: Iron supplementation may reduce postoperative anemia, blood transfusions, and infections in patients undergoing surgery. We sought to assess efficacy and safety of prophylactic intravenous iron supplementation in patients without anemia undergoing cardiac surgery.

Methods: In this investigator-initiated industry-sponsored single-center randomized double-blind parallel group trial, we enrolled patients undergoing coronary bypass, aortic or mitral valve or ascending aortic surgery who fulfilled prespecified iron blood test safety criteria.

Impact of Sheath Type on Vascular and Bleeding Complications After Transcatheter Aortic Valve Replacement: A Post Hoc Analysis From the MARVEL Registry Study.

Background: Vascular and bleeding complications remain a concern after transfemoral transcatheter aortic valve replacement (TAVR). The impact of the sheath type on these complications remains unclear.

Methods: The prospective MARVEL registry study analyzed enrolled 500 patients undergoing large-bore transfemoral procedures and arteriotomy closure with the MANTA vascular closure device from 10 hospitals in Europe and Canada.

Short- and long-term effects of remote synchronous fine-tuning for first-time hearing aid users: a randomised controlled trial.

Objective: To evaluate the short- and long-term effect of remote synchronous fine-tuning and follow-up visits on hearing-related problems and hearing aid (HA) benefits for first-time HA users.

Design: A randomised controlled trial.

Study Sample: Patients at public tax-funded HA clinics in Sweden due for aural rehabilitation (AR) were randomised to either an intervention group (n = 33) or a control group (n = 36).