[The immunogenic and serological properties of the O-specific polysaccharide (L-hapten) in Shigella].

O-specific polysaccharide (L-hapten) was isolated earlier (Zh. mikrobiol. epidemiol.

[A comparison of the sorption capability and standardization of plates of different shapes for immunoenzyme analysis].

The sorption characteristics of ELISA plates manufactured by the Moscow and Leningrad plants and by some foreign firms are compared. Sorption activities and standard specifications of the plates for chemically heterogeneous antigens like ovalbumin or Shigella sonnei lipopolysaccharides and for complex antigens like Mycobacterium tuberculosis or S. sonnei ribosomal fractions have been examined.

[Isotypic characteristics of serum and secretory O antibodies and local immunological memory in the parenteral immunization of guinea pigs with a ribosomal Shigella vaccine].

Guinea pigs were immunized subcutaneously with ribosomal vaccine prepared from S. sonnei and their systemic and local humoral response was studied by means of ELISA techniques with the use of monospecific antisera to guinea pig IgA and IgG. Injection of the ribosomal vaccine leads to a significant rise in the levels of IgA O-antibodies in tears, IgG and IgA O-antibodies in the serum.

[Preparation of monospecific antisera to IgA in laboratory animals using cascade immunization without preliminary isolation of the antigen].

The proposed method of "cascade" immunization for the preparation of monospecific antisera to IgA of the experimental animals (mice, rats, guinea-pigs) requires no isolation and purification of an antigen, since it is based on using precipitation lines containing IgA. The subsequent steps of the method are the following: 1) preparation of a polyspecific antiserum against one of the secretions or IgA-containing serum fraction; 2) preparation of precipitation lines using this antiserum and some other secretions; 3) immunization of other rabbits with these precipitation lines to obtain monospecific anti-IgA antiserum. The antisera obtained do not require any additional absorption except for the removal of anti-IgA antibodies.