Thermodynamic properties of DNA-dendrimer complexes and features of their applications.

The commercial solution of fourth generation (G4) of poly-amidoamine (PAMAM) dendrimers contains methanol, which is toxic for human body. Our differential scanning calorimetry (DSC) study of dendrimers confirmed the existence of this threat. The recommendation is done on how to prepare dendrimer solutions for practical and safely use in gene delivery.

Features of membrane receptors in bacterial multiplication process and necessary conditions for phage infection of bacteria.

According to the obtained experimental results, the thermal shock (from 37 to 53 °C) not only stops the multiplication process of Escherichia coli bacteria, but also causes bacterial titer to decrease gradually. After this period lasting up to 1 hour, the bacterial cells continue to grow. A similar type of response was observed when bacteria were subjected to acid shock.

Structure of drug delivery DPPA and DPPC liposomes with ligands and their permeability through cells.

Dipalmitoylphosphatidylcholine (DPPC) and 1,2-palmitoyl-phosphatidic acid (DPPA) liposomes, prepared by conventional rotary evaporation method, have similar structural organization, though they have significant differences. The similarity is that both types of lipids create standard bilayer liposomes with strong hydrophobic forces between lipids tails and with homogeneous bonds of hydrogen and electrostatic nature between hydrophilic lipids heads. By the calorimetric method, it has been shown that hydrophobic bonds break but liposomes' destruction does not occur by heating till 150 °C.

Study of environmental and antimicrobial agents impact on features of bacterial growth.

Continuous, real-time observation of bacterial growth has a great advantage for studying the mechanisms of interactions of various compounds with the bacterial cell membrane. With the use of physical methods, which are specific for assessment of continuous changes in turbidity over time, we have shown that bacterial growth was affected by not only on types of antibiotics and phages, but also by their concentration in media. Low concentration of antibiotics and bacteriophages in media has no effect on the bacterial growth process.

Expansion of CD3/CD16/CD56 positive NKT cells in HIV/AIDS: the pilot study.

Unlabelled: NKT cells are a subset of lymphocytes possessing features of NK cells and T cells; they play a key role in the formation of innate immune response. Upon stimulation, rapid production of large quantities of both T(h1) and T(h2) type cytokines permits them to bridge the innate and adaptive immune responses by activating NK cells, T cells, B cells and dendritic cells. Scientific knowledge has been collecting up to date toward the definition of the role of NKT lymphocytes in HIV/AIDS setting.