Publications by authors named "M M Hosamani"
Article Synopsis
- * ELISA has been suggested as an easier alternative but struggles with antigen availability, particularly RABV glycoprotein, which requires high biosafety levels for use.
- * The research tested insect cell extracts producing RABV glycoprotein and found it effectively detected rabies antibodies in vaccinated dogs, with a 90.48% sensitivity and 80.00% specificity, making it suitable for quick screening and monitoring vaccination efforts.
The cross-species transmissibility of SARS-CoV-2 infection has necessitated development of specific reagents for detecting infection in various animal species. The spike glycoprotein of SARS-CoV-2, which is involved in viral entry, is a highly immunogenic protein. To develop assays targeting this protein, we generated eight monoclonal antibodies (mAbs) against the S1 and seven against the S1/S2 protein (ectodomain) of SARS CoV-2.
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- This study investigates how lanthanide compounds interact with gamma rays and their buildup factors when exposed to low-energy radioactive sources.
- Researchers used specific experimental setups to measure gamma-ray intensities, finding that their results align closely with theoretical models.
- The findings suggest significant implications for medical diagnostics and innovative nuclear technologies, with insights into how deep gamma rays penetrate these materials based on different analysis methods.
Classical swine fever (CSF) is an economically important and highly contagious disease of pigs caused by CSF virus, genus Pestivirus. Serological diagnosis of the disease is highly valuable for surveillance and thereby containment of spread of the disease. In this study, we have demonstrated the development of CSFV envelope glycoprotein E2-based indirect ELISA (E2-iELISA) for the detection of CSFV specific antibodies.
View Article and Find Full Text PDFDiagnostics employing multiple modalities have been essential for controlling and managing COVID-19, caused by SARS-CoV-2. However, scaling up Reverse Transcription-Quantitative Polymerase Chain Reaction (RT-qPCR), the gold standard for SARS-CoV-2 detection, remains challenging in low and middle-income countries. Cost-effective and high-throughput alternatives like enzyme-linked immunosorbent assay (ELISA) could address this issue.
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