[Effect of hyperbaric oxygenation on proton ATPase activity in mitochondria of various rat tissues].

The H+-ATPase activity of mitochondria from the rat brain, liver heart and skeletal muscles was studied as affected by 1 at. of oxygen pressure (60 min), 5 at. (10 min) and 5 at.

[Peptide hydrolase activity of blood serum, myocardium and skeletal muscles of rats under different states of hyperbaric oxygenation].

Intensity of autolysis and proteolysis (hemoglobin as a substrate) of blood serum, myocardium and skeletal muscles was determined under normal conditions and under the effect of 2.0, 3.5 at.

[Lipid oxidation processes in different rat tissues in the presence of hyperoxia and in the posthyperoxic period].

Oxygen under 5 aim (convulsant and terminal states) evoked an increase in the level of lipid peroxides in the myocardium, spleen, blood, skeletal muscles, brain, kidneys, and liver. In the posthyperoxic period, there was a relatively rapid return to normal of the level of lipid peroxides first in the brain (after 1 hour) and then in other tissues (after 24 or 72 hours).

[Effect of exogenous superoxide dismutase on lipid peroxidation in tissue homogenates under hyperoxia].

Single intravenous administration of the purified preparation of superoxide dismutase did not protect animals from oxygen impairment but decreased distinctly the lipid peroxidation in tissue homogenates (except of brain) in vitro. Addition of the superoxide dismutase preparation into homogenates decreased markedly the alonic dialdehyde accumulation in all the tissues studied, including the brain tissue.

[ATPase activity in neuronal and glial enriched fractions of cerebral cortex in normal conditions and with hyperoxia].

The ATP-hydrolyzing activity was studied without addition of the exogenic cations and in the presence of Ca2+, Mg2+, Na+, K+ in myelin of rat brain cortex fractions enriched with neurons and glia. In the variant without addition of the exogenic cations the fraction enriched with neurons possesses the highest specific activity of ATPase and the myelin fraction possesses the lowest one. Addition of Ca2+, Mg2+, Na+, K+, triton X-100 activates ATPase in the nerve tissue cell fractions, ouabain inhibits the Mg2+, Na+, K+-ATPase activity.